• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脊髓灰质炎病毒RNA上游AUG密码子的突变分析。

Mutational analysis of upstream AUG codons of poliovirus RNA.

作者信息

Pelletier J, Flynn M E, Kaplan G, Racaniello V, Sonenberg N

机构信息

Department of Biochemistry, McGill University, Montreal, Quebec, Canada.

出版信息

J Virol. 1988 Dec;62(12):4486-92. doi: 10.1128/JVI.62.12.4486-4492.1988.

DOI:10.1128/JVI.62.12.4486-4492.1988
PMID:2846865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253558/
Abstract

The 5' untranslated region of poliovirus type 2 Lansing RNA consists of 744 nucleotides containing seven AUG codons which are followed by in-frame termination codons, thus forming short open reading frames (ORFs). To determine the biological significance of these small ORFs, all of the upstream AUG codons were mutated to UUG. The point mutations were introduced into an infectious poliovirus cDNA clone, and RNA transcribed in vitro from the altered cDNA was transfected into HeLa cells to recover the virus. Mutation of AUG 7 resulted in a virus (called R2-5NC-14) with a small-plaque phenotype, whereas mutation of the other six AUG codons produced virus with a wild-type plaque morphology. To determine whether the small-plaque phenotype of R2-5NC-14 was due to altered translational efficiency of the viral mRNA, we constructed chimeric mRNAs containing the 5' noncoding region of poliovirus mRNA fused to the chloramphenicol acetyltransferase (CAT) coding sequence. mRNA containing a mutated AUG 7 codon showed decreased translational efficiency in vitro. The results indicate that the upstream ORFs of poliovirus RNA are not essential for viral replication and do not act as barriers to the translation of poliovirus mRNA. AUG 7 and flanking sequences may play a positive acting role in poliovirus RNA translation.

摘要

2型兰辛株脊髓灰质炎病毒RNA的5'非翻译区由744个核苷酸组成，包含7个AUG密码子，其后是框内终止密码子，从而形成短开放阅读框（ORF）。为了确定这些小ORF的生物学意义，所有上游AUG密码子均突变为UUG。将点突变引入感染性脊髓灰质炎病毒cDNA克隆，将从改变后的cDNA体外转录的RNA转染到HeLa细胞中以回收病毒。AUG 7突变产生了一种具有小噬斑表型的病毒（称为R2-5NC-14），而其他六个AUG密码子的突变产生了具有野生型噬斑形态的病毒。为了确定R2-5NC-14的小噬斑表型是否是由于病毒mRNA翻译效率的改变，我们构建了嵌合mRNA，其包含与氯霉素乙酰转移酶（CAT）编码序列融合的脊髓灰质炎病毒mRNA的5'非编码区。含有突变的AUG 7密码子的mRNA在体外显示出翻译效率降低。结果表明，脊髓灰质炎病毒RNA的上游ORF对病毒复制不是必需的，并且不充当脊髓灰质炎病毒mRNA翻译的障碍。AUG 7及其侧翼序列可能在脊髓灰质炎病毒RNA翻译中起积极作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/1a7cf4714ccc/jvirol00091-0066-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/24b529957f15/jvirol00091-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/263e1f0c9ec8/jvirol00091-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/2292e0f5a744/jvirol00091-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/1a7cf4714ccc/jvirol00091-0066-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/24b529957f15/jvirol00091-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/263e1f0c9ec8/jvirol00091-0065-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/2292e0f5a744/jvirol00091-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/95dd/253558/1a7cf4714ccc/jvirol00091-0066-b.jpg

相似文献

1
Mutational analysis of upstream AUG codons of poliovirus RNA.脊髓灰质炎病毒RNA上游AUG密码子的突变分析。
J Virol. 1988 Dec;62(12):4486-92. doi: 10.1128/JVI.62.12.4486-4492.1988.
2
Effect of mutations downstream of the internal ribosome entry site on initiation of poliovirus protein synthesis.内部核糖体进入位点下游突变对脊髓灰质炎病毒蛋白质合成起始的影响。
J Virol. 1994 Oct;68(10):6312-22. doi: 10.1128/JVI.68.10.6312-6322.1994.
3
Structural and functional analysis of the ribosome landing pad of poliovirus type 2: in vivo translation studies.2型脊髓灰质炎病毒核糖体着陆平台的结构与功能分析：体内翻译研究
J Virol. 1991 Nov;65(11):5886-94. doi: 10.1128/JVI.65.11.5886-5894.1991.
4
Effect of intercistronic length on internal ribosome entry site (IRES) efficiency in bicistronic mRNA.顺反子间长度对双顺反子mRNA中内部核糖体进入位点（IRES）效率的影响。
Gene Expr. 1999;8(5-6):299-309.
5
An RNA hairpin at the extreme 5' end of the poliovirus RNA genome modulates viral translation in human cells.脊髓灰质炎病毒RNA基因组5'端极端位置的一个RNA发夹结构可调节人类细胞中的病毒翻译。
J Virol. 1991 Feb;65(2):913-21. doi: 10.1128/JVI.65.2.913-921.1991.
6
In vitro mutational analysis of cis-acting RNA translational elements within the poliovirus type 2 5' untranslated region.脊髓灰质炎病毒2型5'非翻译区内顺式作用RNA翻译元件的体外突变分析。
J Virol. 1991 Nov;65(11):5895-901. doi: 10.1128/JVI.65.11.5895-5901.1991.
7
Role of the pyrimidine-rich tract on the translation of 'chimeric' polio-hepatitis A mRNAs with engineered 5'-terminal untranslated regions.富含嘧啶序列在具有工程化5'-末端非翻译区的“嵌合”脊髓灰质炎-甲型肝炎mRNA翻译中的作用。
Virus Res. 1995 Aug;37(3):291-303. doi: 10.1016/0168-1702(95)00030-t.
8
Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.脊髓灰质炎病毒mRNA的翻译效率：定位5'非编码区内的抑制性顺式作用元件。
J Virol. 1988 Jul;62(7):2219-27. doi: 10.1128/JVI.62.7.2219-2227.1988.
9
Identification of an alternative open reading frame ("hidden gene"?) stringently required for infectivity of poliovirus cDNA clones.鉴定脊髓灰质炎病毒cDNA克隆感染性严格所需的一个替代性开放阅读框（“隐藏基因”？）
New Microbiol. 1998 Oct;21(4):309-20.
10
Minimum internal ribosome entry site required for poliovirus infectivity.脊髓灰质炎病毒感染所需的最小内部核糖体进入位点。
J Virol. 1993 Dec;67(12):7461-71. doi: 10.1128/JVI.67.12.7461-7471.1993.

引用本文的文献

1
Exploring Virus Diversity in the (), an Economically Important Agricultural Pest.探究（）中的病毒多样性，（）是一种具有重要经济意义的农业害虫。
Viruses. 2024 Aug 16;16(8):1305. doi: 10.3390/v16081305.
2
RNA Structure in the 5' Untranslated Region of Enterovirus D68 Strains with Differing Neurovirulence Phenotypes.肠道病毒 D68 不同神经毒力表型株 5'非翻译区的 RNA 结构。
Viruses. 2023 Jan 20;15(2):295. doi: 10.3390/v15020295.
3
Picornavirus translation strategies.小核糖核酸病毒翻译策略。

本文引用的文献

1
Inactivation of cap-binding proteins accompanies the shut-off of host protein synthesis by poliovirus.帽结合蛋白的失活伴随着脊髓灰质炎病毒对宿主蛋白质合成的关闭。
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3447-51. doi: 10.1073/pnas.79.11.3447.
2
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。
Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.
3
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
FEBS Open Bio. 2022 Jun;12(6):1125-1141. doi: 10.1002/2211-5463.13400. Epub 2022 Mar 30.
4
RNA Binding Proteins as Pioneer Determinants of Infection: Protective, Proviral, or Both?RNA 结合蛋白作为感染的先驱决定因素：保护、促进病毒还是两者兼有？
Viruses. 2021 Oct 28;13(11):2172. doi: 10.3390/v13112172.
5
Translational Control of the Picornavirus Phenotype.微小核糖核酸病毒表型的翻译控制
Mol Biol. 2001;35(4):591-599. doi: 10.1023/A:1010531228348.
6
Human cytomegalovirus pTRS1 stimulates cap-independent translation.人巨细胞病毒 pTRS1 刺激帽非依赖性翻译。
Virology. 2019 Nov;537:246-253. doi: 10.1016/j.virol.2019.08.026. Epub 2019 Aug 29.
7
Structure of the 5' Untranslated Region of Enteroviral Genomic RNA.肠道病毒基因组 RNA 5'非翻译区结构。
J Virol. 2019 Nov 13;93(23). doi: 10.1128/JVI.01288-19. Print 2019 Dec 1.
8
An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells.肠道病毒中的上游蛋白编码区调节肠道上皮细胞中的病毒感染。
Nat Microbiol. 2019 Feb;4(2):280-292. doi: 10.1038/s41564-018-0297-1. Epub 2018 Nov 26.
9
Cellular Proteins Act as Bridge Between 5' and 3' Ends of the Coxsackievirus B3 Mediating Genome Circularization During RNA Translation.细胞蛋白在柯萨奇病毒B3的RNA翻译过程中充当5'端和3'端之间的桥梁，介导基因组环化。
Curr Microbiol. 2015 Sep;71(3):387-95. doi: 10.1007/s00284-015-0866-y. Epub 2015 Jul 3.
10
Intergenic sequence between Arabidopsis caseinolytic protease B-cytoplasmic/heat shock protein100 and choline kinase genes functions as a heat-inducible bidirectional promoter.拟南芥酪蛋白水解蛋白酶B-细胞质/热激蛋白100与胆碱激酶基因之间的基因间序列作为热诱导双向启动子发挥作用。
Plant Physiol. 2014 Nov;166(3):1646-58. doi: 10.1104/pp.114.250787. Epub 2014 Oct 3.
原核生物、真核生物和细胞器中蛋白质合成起始的比较。
Microbiol Rev. 1983 Mar;47(1):1-45. doi: 10.1128/mr.47.1.1-45.1983.
4
Identification of the SV40 agnogene product: a DNA binding protein.猿猴病毒40（SV40）反式激活基因产物的鉴定：一种DNA结合蛋白。
Nature. 1981 May 28;291(5813):346-9. doi: 10.1038/291346a0.
5
Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.所有三种脊髓灰质炎病毒血清型基因组的完整核苷酸序列。对遗传关系、基因功能和抗原决定簇的意义。
J Mol Biol. 1984 Apr 25;174(4):561-85. doi: 10.1016/0022-2836(84)90084-6.
6
Extraction of nucleic acids from agarose gels.从琼脂糖凝胶中提取核酸。
Anal Biochem. 1980 Apr;103(2):264-71. doi: 10.1016/0003-2697(80)90266-3.
7
Translational efficiency of polycistronic mRNAs and their utilization to express heterologous genes in mammalian cells.多顺反子mRNA的翻译效率及其在哺乳动物细胞中表达异源基因的应用。
EMBO J. 1987 Jan;6(1):187-93. doi: 10.1002/j.1460-2075.1987.tb04737.x.
8
Multiple upstream AUG codons mediate translational control of GCN4.多个上游AUG密码子介导GCN4的翻译控制。
Cell. 1986 Apr 25;45(2):201-7. doi: 10.1016/0092-8674(86)90384-3.
9
A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans.一种脊髓灰质炎病毒神经毒力的小鼠模型鉴定出了使该病毒对人类减毒的突变。
J Virol. 1987 Sep;61(9):2917-20. doi: 10.1128/JVI.61.9.2917-2920.1987.
10
Regulation of translation by poliovirus.脊髓灰质炎病毒对翻译的调控
Adv Virus Res. 1987;33:175-204. doi: 10.1016/s0065-3527(08)60318-8.