van Oostende-Triplet Chloë, Guillet Dominique, Triplet Thomas, Pandzic Elvis, Wiseman Paul W, Geitmann Anja
Institut de Recherche en Biologie Végétale, Université de Montréal, Montreal, Quebec H1X 2B2, Canada.
Departments of Physics and Chemistry, McGill University, Montreal, Quebec H3A 2T8, Canada.
Plant Physiol. 2017 Jul;174(3):1544-1558. doi: 10.1104/pp.17.00343. Epub 2017 May 4.
Cell division in plant cells requires the deposition of a new cell wall between the two daughter cells. The assembly of this plate requires the coordinated movement of cargo vesicles whose size is below the diffraction-limited resolution of the optical microscope. We combined high spatial and temporal resolution confocal laser scanning microscopy with advanced image-processing tools and fluorescence fluctuation methods and distinguished three distinct phases during cell plate expansion in tobacco () 'Bright Yellow-2' cells: massive delivery of preexisting vesicles to a disk-shaped region at the equatorial plane precedes a primary rapid expansion phase followed by a secondary, slow expansion phase during which the extremity of the circular plate seeks contact with the mother wall and brings about the separation of the two portions of cytoplasm. Different effects of pharmacological inhibition emphasize the distinct nature of the assembly and expansion mechanisms characterizing these phases.
植物细胞中的细胞分裂需要在两个子细胞之间沉积新的细胞壁。这个细胞板的组装需要货物囊泡的协调运动，这些囊泡的大小低于光学显微镜的衍射极限分辨率。我们将高空间和时间分辨率的共聚焦激光扫描显微镜与先进的图像处理工具和荧光波动方法相结合，区分了烟草（Nicotiana tabacum）“Bright Yellow-2”细胞中细胞板扩展过程的三个不同阶段：大量预先存在的囊泡被输送到赤道平面的盘状区域，随后是主要的快速扩展阶段，接着是次要的缓慢扩展阶段，在此期间圆形细胞板的边缘寻找与母细胞壁接触并导致两部分细胞质分离。药理学抑制的不同作用强调了表征这些阶段的组装和扩展机制的独特性质。
