Vet Res Forum. 2017 Winter;8(1):49-54. Epub 2017 Mar 15.
In this study the optimal amount of enriching with a synbiotic combination of and fructooligosaccharide (FOS( was investigated. The experiment was conducted in a completely randomized design in ten treatments: multi-level probiotics (1×10, 1×10 and 1× 10 CFU per L) and multi-level enriched prebiotic FOS (1, 2 and 5) g per L of solution and control. To evaluate the enrichment of adult artemia with each treatment, sampling was performed at 2, 4 and 6 hr after initiation of enrichment. The results showed that a synbiotic containing a probiotic treatments at 1 × 10 and 1 × 10 CFU per L had more bacteria than a synbiotic containing a probiotic treatment with 1 × 10 CFU per L ( < 0.05), but did not show significant difference between levels of 1 × 10 and 1 × 10 CFU per L ( > 0.05). The highest number of attached bacteria (6.78 ± 0.03 log CFU g1) to adult artemia was shown after 6 hr of enrichment that showed significant difference with 2 hr, but did not show significant difference with 4 hr time. In conclusion, the results of this study showed that adult artemia in a short time (about 4 hr) unlike nauplii artemia can retain a large amount of probiotic (1 × 10 CFU per L and 5 g per L FOS prebiotic) on their own.
在本研究中，对与低聚果糖（FOS）的合生元组合进行富集的最佳量进行了研究。实验采用完全随机设计，设置了十种处理：多级益生菌（每升1×10、1×10和1×10 CFU）、多级富集益生元FOS（每升溶液1、2和5克）以及对照。为评估每种处理对成年卤虫的富集效果，在富集开始后2、4和6小时进行采样。结果表明，每升含1×10和1×10 CFU益生菌处理的合生元比每升含1×10 CFU益生菌处理的合生元含有更多细菌（P<0.05），但每升1×10和1×10 CFU水平之间未显示出显著差异（P>0.05）。富集6小时后，成年卤虫附着细菌数量最多（6.78±0.03 log CFU g1），与2小时相比有显著差异，但与4小时无显著差异。总之，本研究结果表明，成年卤虫与无节幼体卤虫不同，能在短时间内（约4小时）自行保留大量益生菌（每升1×10 CFU和每升5克FOS益生元）。
