蓝舌病病毒（BTV）血清型17全长基因组片段2、5、6和8的克隆及其与美国BTV血清型的遗传相关性研究。
The cloning of full-length genome segments 2, 5, 6, and 8 of bluetongue virus (BTV) serotype 17 and studies of their genetic relatedness to United States BTV serotypes.
作者信息
Unger R E, Chuang R Y, Chuang L F, Osburn B I, Doi R H
机构信息
Department of Medical Pharmacology, University of California, Davis.
出版信息
Virology. 1988 Nov;167(1):296-8. doi: 10.1016/0042-6822(88)90083-9.
The double-stranded (ds) RNA genome segments 2, 5, 6, and 8, which encode the outer capsid proteins P2 and P5, and the two nonstructural proteins, NS1 and NS2, respectively, of bluetongue virus (BTV) serotype 17 have been cloned into pBR322. The length of the cloned genes indicates that the entire respective dsRNA genome has been cloned in each case. The four cloned genes were used as 32P-labeled probes to hybridize to PAGE-separated United States BTV serotypes 2, 10, 11, 13, and 17. Of these, the segment 6 clone was identified as being highly conserved and hybridized to all serotypes. Segment 8 was less conserved and segment 5 even less. The segment 2 clone was serotype specific. None of the probes hybridized with dsRNA from epizootic hemorrhagic disease virus (EHDV).
蓝舌病病毒17型的双链（ds）RNA基因组片段2、5、6和8分别编码病毒外衣壳蛋白P2和P5以及两种非结构蛋白NS1和NS2，它们已被克隆到pBR322中。克隆基因的长度表明在每种情况下均已克隆了完整的相应dsRNA基因组。这四个克隆基因用作32P标记的探针，与经聚丙烯酰胺凝胶电泳（PAGE）分离的美国蓝舌病病毒2型、10型、11型、13型和17型进行杂交。其中，片段6克隆被鉴定为高度保守，与所有血清型均发生杂交。片段8的保守性较差，片段5的保守性更差。片段2克隆具有血清型特异性。没有一种探针能与流行性出血病病毒（EHDV）的dsRNA杂交。
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