Druzhko Anna B, Dyukova Tatyana V, Pirutin Sergey K
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow region, 142290, Russia.
Biophysical Department, Biology Faculty, Lomonosov Moscow State University, Moscow, Russia.
Eur Biophys J. 2017 Sep;46(6):509-515. doi: 10.1007/s00249-017-1211-0. Epub 2017 May 4.
This review presents the results of studies concerning some factors that affect the process of photoinduced hydroxylaminolysis (PHA) in bacteriorhodopsin (BR) and different BR-based media. We consider the peculiar properties of the PHA reaction in water suspensions of BR and BR-based media depending on variation in PHA ingredients, and in particular the use of O-substituted hydroxylamines instead of hydroxylamine hydrochloride. In addition, we discuss how such factors as preliminary ultra-sonication affect the reaction of PHA in the course of BR bleaching and following the reconstitution of bacterioopsin. All the results are considered from the viewpoint of improving the performance of BR-based media as photosensitive materials for the processing and storage of optical information.
本综述介绍了有关一些影响细菌视紫红质(BR)及不同基于BR的介质中光诱导羟胺分解(PHA)过程的因素的研究结果。我们考虑了BR和基于BR的介质的水悬浮液中PHA反应的特殊性质,这取决于PHA成分的变化,特别是使用O-取代羟胺代替盐酸羟胺的情况。此外,我们还讨论了诸如初步超声处理等因素如何在BR漂白过程中以及细菌视蛋白重构后影响PHA反应。所有结果均从提高基于BR的介质作为用于光学信息处理和存储的光敏材料的性能这一角度进行考量。