Loftin K C, Gonik B, Kumaran P
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas Medical School, Houston 77030.
Am J Reprod Immunol Microbiol. 1988 Jun;17(2):53-6. doi: 10.1111/j.1600-0897.1988.tb00202.x.
In vitro natural killer cytotoxicity (NKC) and antibody-dependent cell cytotoxicity (ADCC) activity against herpes simplex virus (HSV)-infected cells were evaluated in a pregnant murine model (C57Bl6 inbred strain). Virgin (n = 16) and pregnant (late gestation) mice (n = 15) were infected intraperitoneally with HSV, type 1. After 18 hr, a 0.5-ml aliquot of the peritoneal wash was frozen for virus plaque assay, and the cells were cultured in the 51chromium release assay for NKC and ADCC. %NKC (mean +/- S.E.) to HSV-infected targets was significantly suppressed (P less than 0.05) in pregnant mice, 10.3% +/- 1.9, compared to that of virgin mice, 32.5% +/- 2.5. This suppression was abrogated with HSV-specific antisera (%ADCC); 53.9% +/- 4.4 (pregnant) compared to 49.1% +/- 3.6 (virgin). The diminished NKC activity in pregnant mice was reflected in an increased mean number of virus particles in the peritoneal wash, 266 +/- 66 PFU/ml, compared to 38 +/- 11 PFU/ml in virgin mice (P less than 0.05). We concluded that NKC, but not ADCC, to HSV-infected targets was suppressed and that HSV elimination was impaired in pregnant mice.
在一个怀孕小鼠模型（C57Bl6近交系）中评估了针对单纯疱疹病毒（HSV）感染细胞的体外自然杀伤细胞细胞毒性（NKC）和抗体依赖性细胞毒性（ADCC）活性。将未孕（n = 16）和怀孕（妊娠晚期）小鼠（n = 15）腹腔内感染1型HSV。18小时后，取0.5 ml腹腔洗液冻存用于病毒空斑试验，细胞用于51铬释放试验以检测NKC和ADCC。与未孕小鼠（32.5%±2.5）相比，怀孕小鼠对HSV感染靶标的%NKC（平均值±标准误）显著受到抑制（P<0.05），为10.3%±1.9。用HSV特异性抗血清（%ADCC）可消除这种抑制；怀孕小鼠为53.9%±4.4，未孕小鼠为49.1%±3.6。怀孕小鼠NKC活性降低反映在腹腔洗液中病毒颗粒平均数量增加，为266±66 PFU/ml，而未孕小鼠为38±11 PFU/ml（P<0.05）。我们得出结论，怀孕小鼠对HSV感染靶标的NKC受到抑制，而ADCC未受抑制，且HSV清除受损。
