Scott T W, Olson J G, All B P, Gibbs E P
Department of Entomology, University of Maryland, College Park 20742.
Am J Vet Res. 1988 Oct;49(10):1716-8.
Sensitivity and specificity of an antigen-capture ELISA vs virus isolation in cell culture were evaluated for the detection of eastern equine encephalomyelitis (EEE) virus in the brain tissue of naturally infected equids. Brain specimens from 16 equids with neurologic disease were examined by ELISA and by inoculation onto baby hamster kidney cell cultures. Of 10 brain samples from which virus was isolated in the cell culture bioassay, all were correctly identified as containing EEE virus antigen by ELISA. None of the remaining 6 specimens, without detectable infectious EEE virus, contained detectable antigen. Sensitivity and specificity of the ELISA were 100% with no false-positive or false-negative results. The antigen-capture ELISA was a rapid, sensitive, specific, and simple alternative to a traditional bioassay for the detection of EEE virus.
评估了抗原捕获酶联免疫吸附测定（ELISA）与细胞培养中病毒分离法检测自然感染马属动物脑组织中东部马脑脊髓炎（EEE）病毒的敏感性和特异性。对16例患有神经疾病的马属动物的脑标本进行了ELISA检测，并接种到幼仓鼠肾细胞培养物中。在细胞培养生物测定中分离出病毒的10个脑样本中，通过ELISA均正确鉴定为含有EEE病毒抗原。其余6个未检测到传染性EEE病毒的标本中，均未检测到抗原。ELISA的敏感性和特异性均为100%，无假阳性或假阴性结果。抗原捕获ELISA是一种快速、灵敏、特异且简单的传统生物测定替代方法，用于检测EEE病毒。
