Isolation of organic compounds with high added values from agro-industrial solid wastes.

Phenols are organic compounds with high antioxidant activity. Occurring mainly in plants, where they act as pigments or even as part of defense mechanisms against insects and herbivores. Given the positive impact on on human health, their isolation and purification from agricultural products is of particular interest for the production of nutritional, pharmaceutical and cosmetics supplements. In our study different materials rich in phenolic compounds were used, in order to separate the phenolic content and maximum condensation using physicochemical methods such as solvent extraction, filtration through membranes, adsorption/desorption on resins and vacuum distillation. The materials tested were solid wastes from winery, cocoa residuals, olive leaves, etc. The first step for the treatment was the extraction of phenolic content using water-ethanol solutions which was initially optimized. Then, sequential membrane filtration of the extracts by Ultrafiltration membranes, Nanofiltration and Reverse Osmosis was performed to separate the contained compounds, based on their molecular weight. To remove non-polar compounds, with similar molecular weights with phenols, methods of adsorption/desorption on specific resins were developed, in order final ethanolic solutions rich in phenolic compounds to be obtained. Finally, the ethanol was removed by vacuum evaporation at low temperatures. The purification of olive leaf phenols is illustrated in details in the present work. The final obtained concentrate, was a rich phenolic concentrate and contained 98 g/L phenols in gallic acid equivalents. This technique, after modification, can be applied to a variety of phenol-rich byproducts, allowing the operation of phenol separation plant adjustable to local agricultural activities.