从日本男性尿道和咽部分离出的沙眼衣原体的抗菌药敏性。
Antimicrobial susceptibilities of Chlamydia trachomatis isolated from the urethra and pharynx of Japanese males.
作者信息
Kai Seiji, Wada Koichiro, Sadahira Takuya, Araki Motoo, Ishii Ayano, Watanabe Toyohiko, Monden Koichi, Uno Satoshi, Araki Tohru, Nasu Yasutomo
机构信息
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8558, Japan; Department of Urology, Hiroshima City Hiroshima Citizens Hospital, 7-33, Moto-machi, Naka-ku, Hiroshima, 730-8518, Japan; Okayama Urological Research Group (OURG), 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.
Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8558, Japan; Okayama Urological Research Group (OURG), 2-5-1, Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.
出版信息
J Infect Chemother. 2017 Aug;23(8):512-516. doi: 10.1016/j.jiac.2017.04.005. Epub 2017 May 2.
OBJECTIVES
Sexually transmitted infections due to Chlamydia trachomatis (C. trachomatis) are a worldwide public health problem. The aim of this study was to investigate the drug susceptibilities of C. trachomatis strains isolated from the urethra and pharynx of Japanese males.
METHODS
Urethral and pharyngeal swabs were collected between 2013 and 2014 from Japanese males with urethritis. Using a McCoy cell line, 18 chlamydial strains were isolated from urethra in 18 patients and 7 from the pharynx in 7 of the 18 patients. The minimum inhibitory concentrations (MICs) of levofloxacin (LVFX) and azithromycin (AZM) were measured using the standard method of the Japanese Society of Chemotherapy.
RESULTS
The MICs of LVFX and AZM against urethral chlamydial strains were 0.125-0.5 μg/mL and 0.125-0.25 μg/mL, respectively. In pharyngeal strains, the MICs of LVFX and AZM were 0.125-0.25 μg/mL and 0.125-0.25 μg/mL, respectively. In 7 patients with chlamydial strains isolated from both the urethra and pharynx, the MICs of LVFX between these strains were identical in 3 of 6 patients (no growth was observed for one pharyngeal strain), while the MICs of AZM between these strains were identical in all 6 patients (not performed for one patient).
CONCLUSIONS
Our data suggest that C. trachomatis strains isolated from the urethra and pharynx of Japanese males are susceptible to LVFX and AZM. Although measuring the MICs of chlamydial strains is labor intensive, it is a significant surveillance tool for treating chlamydial infections and preventing the spread of STIs.
目的
沙眼衣原体（C. trachomatis）引起的性传播感染是一个全球性的公共卫生问题。本研究的目的是调查从日本男性尿道和咽部分离出的沙眼衣原体菌株的药敏情况。
方法
在2013年至2014年期间，从患有尿道炎的日本男性中采集尿道和咽部拭子。使用 McCoy 细胞系，从18例患者的尿道中分离出18株衣原体菌株，从这18例患者中的7例咽部中分离出7株。采用日本化疗学会的标准方法测定左氧氟沙星（LVFX）和阿奇霉素（AZM）的最低抑菌浓度（MICs）。
结果
LVFX和AZM对尿道衣原体菌株的MICs分别为0.125 - 0.5μg/mL和0.125 - 0.25μg/mL。在咽部菌株中，LVFX和AZM的MICs分别为0.125 - 0.25μg/mL和0.125 - 0.25μg/mL。在7例同时从尿道和咽部分离出衣原体菌株的患者中，6例患者中有3例这些菌株之间LVFX的MICs相同（1例咽部菌株未观察到生长），而6例患者中所有患者这些菌株之间AZM的MICs相同（1例患者未进行检测）。
结论
我们的数据表明，从日本男性尿道和咽部分离出的沙眼衣原体菌株对LVFX和AZM敏感。虽然测定衣原体菌株的MICs labor intensive，但它是治疗衣原体感染和预防性传播感染传播的重要监测工具。
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