Miralles V J, Marcote M J, Hernández-Yago J, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Spain.
Arch Biochem Biophys. 1988 Nov 1;266(2):516-21. doi: 10.1016/0003-9861(88)90284-6.
Rat liver mitochondria were loaded with cytochrome c by incubation with large amounts of [14C]apocytochrome c. After being washed they were incubated with either more apocytochrome c or cytochrome c. There was no release of labeled proteins from the mitochondria when incubated with cytochrome c. However, there was when incubated with apocytochrome c. The material released showed only one radioactive band which migrated as cytochrome c. Also no release of proteins other than cytochrome c was detected when liver mitochondria isolated from rats injected with [35S]methionine were incubated with apocytochrome c. These results suggest that the level and possibly the turnover of cytochrome c in rat liver mitochondria is regulated by the entry of apocytochrome c into mitochondria.
通过与大量[14C]脱辅基细胞色素c孵育，使大鼠肝脏线粒体加载细胞色素c。洗涤后，将它们与更多的脱辅基细胞色素c或细胞色素c一起孵育。当与细胞色素c孵育时，线粒体没有释放标记蛋白。然而，当与脱辅基细胞色素c孵育时则有释放。释放的物质仅显示出一条迁移速度与细胞色素c相同的放射性条带。当将从注射了[35S]甲硫氨酸的大鼠分离的肝脏线粒体与脱辅基细胞色素c孵育时，也未检测到除细胞色素c以外的蛋白质释放。这些结果表明，大鼠肝脏线粒体中细胞色素c的水平以及可能的周转受脱辅基细胞色素c进入线粒体的调节。
