Scocca J R, Hartog K O, Krag S S
Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland.
Biochem Biophys Res Commun. 1988 Nov 15;156(3):1063-9. doi: 10.1016/s0006-291x(88)80740-x.
Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast transferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.
用酵母转移酶基因ALG7对衣霉素抗性中国仓鼠卵巢细胞3E11的基因组DNA进行限制性酶切消化分析。所呈现的数据表明，这些细胞中N-乙酰葡糖胺-1-磷酸转移酶基因发生了中度扩增，这与之前观察到的染色体易位和酶活性增加相一致。这是基因扩增作为N-连接糖基化异常机制的首个实例。
