Mania M, Maruccio L, Russo F, Abbate F, Castaldo L, D'Angelo L, de Girolamo P, Guerrera M C, Lucini C, Madrigrano M, Levanti M, Germanà A
Department of Veterinary Science, University of Messina, Italy.
Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Italy.
Ann Anat. 2017 Jul;212:37-47. doi: 10.1016/j.aanat.2017.03.005. Epub 2017 May 3.
The expression and localization of leptin (A and B) and its receptor family in control and diet-induced obese (DIO) adult male zebrafish gut, after 5-weeks overfeeding, administering Artemia nauplii, as fat-rich food, were investigated. Recently, the obese adult zebrafish was considered an experimental model with pathophysiological pathways similar to mammalian obesity. Currently, there are no reports about leptin in fish obesity, or in a state of altered energy balance. By qRT-PCR, leptin A and leptin B expression levels were significantly higher in DIO zebrafish gut than in the control group (CTRL), and the lowest levels of leptin receptor mRNA appeared in DIO zebrafish gut. The presence of leptin and its receptor proteins in the intestinal tract was detected by western blot analysis in both control and DIO zebrafish. By single immunohistochemical staining, leptin and leptin receptor immunoreactive endocrine cells were identified in the intestinal tract either in DIO or control zebrafish. Moreover, leptin immunopositive enteric nervous system elements were observed in both groups. By double immunohistochemical staining, leptin and its receptor were colocalized especially in DIO zebrafish. Thus, our study represents a starting point in the investigation of a possible involvement of leptin in control of energy homeostasis in control and DIO zebrafish.
研究了在5周过度投喂富含脂肪的食物卤虫无节幼体后，瘦素（A和B）及其受体家族在对照和饮食诱导肥胖（DIO）成年雄性斑马鱼肠道中的表达和定位。最近，肥胖成年斑马鱼被认为是一种具有与哺乳动物肥胖相似病理生理途径的实验模型。目前，尚无关于鱼类肥胖或能量平衡改变状态下瘦素的报道。通过qRT-PCR检测发现，DIO斑马鱼肠道中瘦素A和瘦素B的表达水平显著高于对照组（CTRL），而瘦素受体mRNA水平在DIO斑马鱼肠道中最低。通过蛋白质免疫印迹分析在对照和DIO斑马鱼中均检测到肠道中瘦素及其受体蛋白的存在。通过单免疫组织化学染色，在DIO或对照斑马鱼的肠道中均鉴定出瘦素和瘦素受体免疫反应性内分泌细胞。此外，在两组中均观察到瘦素免疫阳性的肠神经系统成分。通过双免疫组织化学染色，瘦素及其受体尤其在DIO斑马鱼中共定位。因此，我们的研究是调查瘦素可能参与对照和DIO斑马鱼能量稳态控制的起点。
