Errico Sonia, Nicolucci Carla, Migliaccio Marina, Micale Valeria, Mita Damiano G, Diano Nadia
Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Via S. M. di Costantinopoli, 16, 80138 Naples, Italy.
Institute for Coastal marine Environment (IAMC), National Research Council (CNR), Messina, Italy.
Mar Pollut Bull. 2017 Jul 15;120(1-2):68-74. doi: 10.1016/j.marpolbul.2017.04.061. Epub 2017 May 5.
This study reports the occurrence of some endocrine disrupting chemicals in red mullet samples and sediments collected in two representative sites of the northern Sicilian coast (Italy). For this purpose, an improved method, using solid extraction and high-performance liquid chromatography analyses for the simultaneous determination of bisphenol A (BPA), 4-nonylphenol (4-NP) and 4-t-octylphenol (4-t-OP) in fish tissues and sediments, has been developed and validated. Method performance was demonstrated over the concentration range 0.1-200ng/mL, with detection limits from 0.06 to 0.1ng/mL. Recoveries ranged from 83.4% to 102.6%, with relative standard deviations of 7.7-14.0% for the entire procedure. Results showed that BPA, 4-t-OP and 4-NP were detected in all fish samples and sediments from two sampling sites, indicating that these chemicals have contaminated Mediterranean aquatic ecosystem and have accumulated in fish. The study provided more comprehensive fundamental data for risk assessment and contamination control of phenolic EDCs in aquatic environment.
本研究报告了在意大利西西里岛北部海岸两个代表性地点采集的红鲻鱼样本和沉积物中一些内分泌干扰化学物质的存在情况。为此，开发并验证了一种改进方法，该方法采用固相萃取和高效液相色谱分析，用于同时测定鱼类组织和沉积物中的双酚A（BPA）、4-壬基酚（4-NP）和4-叔辛基酚（4-t-OP）。该方法在0.1-200ng/mL的浓度范围内表现良好，检测限为0.06至0.1ng/mL。回收率在83.4%至102.6%之间，整个过程的相对标准偏差为7.7-14.0%。结果表明，在两个采样点的所有鱼类样本和沉积物中均检测到了BPA、4-t-OP和4-NP，这表明这些化学物质已污染地中海水生生态系统并在鱼类中积累。该研究为水生环境中酚类内分泌干扰物的风险评估和污染控制提供了更全面的基础数据。
