[细胞色素P-450 LM2处于可溶状态和膜结合状态时的电子自旋共振光谱分析]
[ESR-spectroscopy of cytochrome P-450 LM2 in the soluble and membrane-bound state].
作者信息
Uvarov V Iu, Davydov R M, Timofeev V P
出版信息
Biofizika. 1988 Jul-Aug;33(4):719-20.
PMID:2847813
Abstract
Influence of microenvironment on the structure of spin-labeled cytochrome P-450 LM2 was studied. The distance between the spin-label which is covalently bound to cysteine-152 and heme ferrum in soluble protein is 17 A and 23 A in the membrane-bound one. The spin-label was exposed in water solution in both cases. It was shown that solubilized cytochrome P-450 LM2 in water solution forms the aggregates consisting of 4-6 monomers.
摘要
研究了微环境对自旋标记细胞色素P-450 LM2结构的影响。共价结合于半胱氨酸-152的自旋标记与可溶性蛋白质中血红素铁之间的距离在膜结合形式中为17埃，在膜结合形式中为23埃。在两种情况下，自旋标记均暴露于水溶液中。结果表明，水溶液中溶解的细胞色素P-450 LM2形成由4-6个单体组成的聚集体。
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