一种基于细胞的荧光检测法，用于检测抑制蛋白质合成的AB毒素的活性。
A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis.
作者信息
Cherubin Patrick, Quiñones Beatriz, Elkahoui Salem, Yokoyama Wallace, Teter Ken
机构信息
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 12722 Research Parkway, Orlando, FL, 32826, USA.
USDA-ARS, Produce Safety and Microbiology Research Unit, Western Regional Research Center, Albany, CA, USA.
出版信息
Methods Mol Biol. 2017;1600:25-36. doi: 10.1007/978-1-4939-6958-6_3.
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.
许多AB毒素会引发一种涉及蛋白质合成抑制的细胞毒性效应。在本章中，我们描述了一种基于细胞的简单荧光测定法，用于检测和量化蛋白质合成的抑制情况。该测定法还可鉴定和表征毒素抑制剂。
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