Cherubin Patrick, Quiñones Beatriz, Elkahoui Salem, Yokoyama Wallace, Teter Ken
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 12722 Research Parkway, Orlando, FL, 32826, USA.
USDA-ARS, Produce Safety and Microbiology Research Unit, Western Regional Research Center, Albany, CA, USA.
Methods Mol Biol. 2017;1600:25-36. doi: 10.1007/978-1-4939-6958-6_3.
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.
许多AB毒素会引发一种涉及蛋白质合成抑制的细胞毒性效应。在本章中，我们描述了一种基于细胞的简单荧光测定法，用于检测和量化蛋白质合成的抑制情况。该测定法还可鉴定和表征毒素抑制剂。
