Chang Shu-Chi, Lu Bo-Li, Lin Jiang-Jen, Li Yen-Hsien, Lee Maw-Rong
Department of Environmental Engineering, National Chung Hsing University, No. 250, Kuo-Kuang Road, Taichung, 40227, Taiwan.
Institute of Polymer Science and Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.
Methods Mol Biol. 2017;1600:85-94. doi: 10.1007/978-1-4939-6958-6_8.
Algal toxin is a unique type of toxin generated with harmful algal blooms in water bodies. This phenomenon is worsened by eutrophication caused by excessive discharge of nutrients into surface water bodies. Since algal toxins are hard to remove after they enter the water treatment processes, an efficient method is required to inhibit the growth of algal cells, to settle the cells at the bottom of the water body and to removes the toxin from the water. We report an efficient way to prepare a novel nanohybrid material, i.e., magnetic nanosilicate platelet (MNSP), and its effects on the removal of microcystin toxins as well as the cells of Microcystis aeruginosa. MNSP was fabricated by a special treatment of a clay mineral, montmorillonite, and then its surface was decorated with magnetite nanoparticles by in situ synthesis. The nanohybrid can efficiently inhibit the growth of M. aeruginosa-a typical species that can generate one of the most notorious algal toxins, i.e., microcystins. Algal cells can be settled with minimal 500 ppm MNSP, and the turbidity can be reduced by more than 67%. The removal of microcystin-LR (MC-LR) was as high as 99.39% at an concentration of 100 ppm, while the pristine nanosilicate platelet could only remove 36.84% at the same dosage.
藻毒素是水体中有害藻华产生的一种独特类型的毒素。由于向地表水体过度排放营养物质导致富营养化，这一现象更加恶化。由于藻毒素进入水处理过程后难以去除，因此需要一种有效的方法来抑制藻细胞的生长，使细胞沉降到水体底部，并从水中去除毒素。我们报告了一种制备新型纳米杂化材料，即磁性纳米硅酸盐片层（MNSP）的有效方法，及其对微囊藻毒素的去除效果以及对铜绿微囊藻细胞的影响。MNSP是通过对一种粘土矿物蒙脱石进行特殊处理制备的，然后通过原位合成在其表面修饰磁铁矿纳米颗粒。这种纳米杂化物可以有效地抑制铜绿微囊藻的生长，铜绿微囊藻是一种典型的能产生最臭名昭著的藻毒素之一，即微囊藻毒素的物种。藻细胞可以在最低500 ppm的MNSP作用下沉降，浊度可以降低67%以上。在100 ppm的浓度下，微囊藻毒素-LR（MC-LR）的去除率高达99.39%，而在相同剂量下，原始的纳米硅酸盐片层只能去除36.84%。
