Flores Sharon R, Hall Elizabeth M, De Jesús Víctor R
Newborn Screening and Molecular Biology Branch, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
Newborn Screening and Molecular Biology Branch, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
Clin Biochem. 2017 Oct;50(15):878-881. doi: 10.1016/j.clinbiochem.2017.05.003. Epub 2017 May 4.
Prior to initial distribution of Glucose-6-phosphate dehydrogenase (G6PD) proficiency testing (PT) materials, we evaluated G6PD enzyme stability in dried blood spots (DBS) under various temperature and humidity environments to develop storage and usage guidelines for our new materials.
DESIGN & METHODS: We prepared fresh G6PD-normal DBS materials and conducted stability evaluations of daily use and short and long-term storage under various temperature and humidity environments.
G6PD DBS PT materials retained 92% of initial activity after 30days of use at 4°C. Materials stored at -20°C and 4°C with desiccant for 30days retained 95% and 90% of initial activity, respectively. When stored for one year at -20°C or six months at 4°C specimens retained >90% of initial activity. Specimens stored at 37°C with desiccant lost 10% activity in three days. At the end of 30days, specimens stored under 'Extreme'-humidity >50% without desiccant- conditions at 37°C assayed below the NSQAP cut off for G6PD. Humidity exacerbated loss of enzyme activity with increasing temperature and time duration.
Data suggest that G6PD PT materials can be stored at 4°C and used for up to one month and can be stored at -20°C for one year and yield >90% enzyme activity. Exposure to warm temperatures, especially with elevated humidity, should be avoided. Desiccant should always be used to mitigate humidity effects.
在首次分发葡萄糖-6-磷酸脱氢酶（G6PD）能力验证（PT）材料之前，我们评估了在各种温度和湿度环境下干燥血斑（DBS）中G6PD酶的稳定性，以制定我们新材料的储存和使用指南。
我们制备了新鲜的G6PD正常DBS材料，并在各种温度和湿度环境下进行了日常使用以及短期和长期储存的稳定性评估。
G6PD DBS PT材料在4°C下使用30天后保留了初始活性的92%。在-20°C和4°C下并使用干燥剂储存30天的材料分别保留了初始活性 的95%和90%。在-20°C下储存一年或在4°C下储存六个月时，标本保留了>90%的初始活性。在37°C下并使用干燥剂储存的标本在三天内损失了10%的活性。在30天结束时，在37°C下“极端”湿度>50%且无干燥剂条件下储存的标本检测结果低于G6PD的NSQAP临界值。湿度随着温度和持续时间的增加加剧了酶活性的损失。
数据表明，G6PD PT材料可在4°C下储存并使用长达一个月，可在-20°C下储存一年且酶活性>90%。应避免暴露在温暖的温度下，尤其是湿度较高时。应始终使用干燥剂来减轻湿度影响。
