血红蛋白 A 和 S 在干燥血斑中的稳定性研究，存储于可控条件下。
Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions.
机构信息
Centers for Disease Control and Prevention (CDC), 4770 Buford Highway NE, Atlanta, GA 30341, USA.
Centers for Disease Control and Prevention (CDC), 4770 Buford Highway NE, Atlanta, GA 30341, USA.
出版信息
Clin Biochem. 2013 Aug;46(12):1089-1092. doi: 10.1016/j.clinbiochem.2013.05.043. Epub 2013 May 20.
Abstract
OBJECTIVE
We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples.
DESIGN AND METHODS
We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run.
RESULTS
During the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage.
CONCLUSIONS
Minimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules.
摘要
目的
我们旨在分别测量热和湿度对干燥血斑（DBS）样本中血红蛋白 A 和 S 水平变化的贡献。
设计和方法
我们将成对的 DBS 样本在低湿度和高湿度环境中于 37°C 下储存预定的时间间隔。在单个高效液相色谱运行中测量每个完整集的所有样本中的血红蛋白 A 和 S 水平。
结果
在一个月的储存间隔期间，在低湿度储存中两种血红蛋白都损失了约初始水平的 35%，而在高湿度储存中几乎损失了初始水平的全部。
结论
在 DBS 样本的运输和储存环境中，最小化湿度和温度对于保持血红蛋白四聚体分子的完整性至关重要。
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