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本文引用的文献

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The stability of markers in dried-blood spots for recommended newborn screening disorders in the United States.美国推荐的新生儿筛查疾病中干血斑标志物的稳定性。
Clin Biochem. 2011 Dec;44(17-18):1445-50. doi: 10.1016/j.clinbiochem.2011.09.010. Epub 2011 Sep 21.
2
The preparation and storage of dried-blood spot quality control materials for lysosomal storage disease screening tests.溶酶体贮积病筛查试验用干血斑质量控制材料的制备和储存。
Clin Biochem. 2011 Jun;44(8-9):704-10. doi: 10.1016/j.clinbiochem.2011.02.014. Epub 2011 Mar 4.
3
Newborn screening fact sheets.新生儿筛查情况说明书。
Pediatrics. 2006 Sep;118(3):e934-63. doi: 10.1542/peds.2006-1783.
4
Development and characterization of dried blood spot materials for the measurement of immunoreactive trypsinogen.用于测量免疫反应性胰蛋白酶原的干血斑材料的开发与特性研究
J Med Screen. 2006;13(2):79-84. doi: 10.1258/096914106777589623.
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Observations on heat/humidity denaturation of enzymes in filter-paper blood spots from newborns.新生儿滤纸血斑中酶的热/湿度变性观察
Clin Chem. 2005 Jun;51(6):1060-2. doi: 10.1373/clinchem.2005.049270. Epub 2005 Apr 21.
7
Validation of accuracy-based amino acid reference materials in dried-blood spots by tandem mass spectrometry for newborn screening assays.串联质谱法对干血斑中基于准确性的氨基酸参考物质用于新生儿筛查检测的验证
Clin Chem. 1999 Aug;45(8 Pt 1):1269-77.
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Sickle-cell trait: benign or malignant?镰状细胞性状：良性还是恶性？
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Red cell life span in sickle cell trait.镰状细胞性状中的红细胞寿命
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Minimizing false positive diagnoses in newborn screening for galactosemia.
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血红蛋白 A 和 S 在干燥血斑中的稳定性研究，存储于可控条件下。

Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions.

机构信息

Centers for Disease Control and Prevention (CDC), 4770 Buford Highway NE, Atlanta, GA 30341, USA.

Centers for Disease Control and Prevention (CDC), 4770 Buford Highway NE, Atlanta, GA 30341, USA.

出版信息

Clin Biochem. 2013 Aug;46(12):1089-1092. doi: 10.1016/j.clinbiochem.2013.05.043. Epub 2013 May 20.

DOI:10.1016/j.clinbiochem.2013.05.043
PMID:23701845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4557610/
Abstract

OBJECTIVE

We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples.

DESIGN AND METHODS

We stored paired sets of DBSs at 37°C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run.

RESULTS

During the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage.

CONCLUSIONS

Minimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules.

摘要

目的

我们旨在分别测量热和湿度对干燥血斑（DBS）样本中血红蛋白 A 和 S 水平变化的贡献。

设计和方法

我们将成对的 DBS 样本在低湿度和高湿度环境中于 37°C 下储存预定的时间间隔。在单个高效液相色谱运行中测量每个完整集的所有样本中的血红蛋白 A 和 S 水平。

结果

在一个月的储存间隔期间，在低湿度储存中两种血红蛋白都损失了约初始水平的 35%，而在高湿度储存中几乎损失了初始水平的全部。

结论

在 DBS 样本的运输和储存环境中，最小化湿度和温度对于保持血红蛋白四聚体分子的完整性至关重要。