Liu Sheng, Wang Xiao-Min, Yang Guo-Wang
Department of Oncology, Beijing Hospital of Traditional Chinese Medicine (TCM) affiliated to Capital Medical University, Beijing 100010, China.
Afr J Tradit Complement Altern Med. 2016 Nov 23;14(1):242-250. doi: 10.21010/ajtcam.v14i1.26. eCollection 2017.
RuanGanJieDu (RGJD) is a traditional Chinese medicine comprising nine Chinese medicinal herbs. The clinical use of RGJD by us was found that it has a good control effect on hepatic fibrosis (HF) patient. In order to further investigate the Anti-fibrosis mechanism of RGJD, hence this experiment has been carried out.
After treatment, the general conditions of rats in each group were observed. The liver tissues morphology and HF phases were observed under light microscope. The weights of livers and spleens were recorded; the indices of liver and spleen were calculated. The α-SMA and E-cadherin of the liver tissues were determined by Immunohistochemistry, and the contents of ALT AST and PCIII+PCIV+HA+LN in rat serum were detected by ELISA.
After treatment, the rats in model group presented with abdominal distention and rough hair, lethargy and mental sluggishness, slow movement and irritability; Most rats in RGJD group and colchicine(Col) group were in good spirits and no obvious abnormal motion state. The RGJD had shown significant effects in improving the liver tissue morphology. The HF phases of RGJD group and Col group mainly distributed in the S1-S2 phases, were finer than the model group in the S3 phase (P<0.05). The wet weights and indices of livers and spleens in RGJD group were lower than the control groups (P<0.05). Moreover, the expressions of E-cadherin, α-SMA and the serum levels of AST, ALT and PCIII+ PCIV in RGJD group were obviously decreased than the control groups except HA and LN(P<0.05).
RGJD can improve the liver tissue morphology and reduce serum biochemical and collagen indices of HF, which proved a better curative effect of Chinese medicine than Col on HF and improving liver function.
软肝解毒（RGJD）是一种由九味中药组成的中药制剂。我们在临床应用中发现，RGJD对肝纤维化（HF）患者有较好的控制效果。为进一步探讨RGJD的抗纤维化机制，故开展本实验。
治疗后观察各组大鼠的一般情况。光镜下观察肝组织形态及肝纤维化分期。记录肝脏和脾脏重量，计算肝脾指数。采用免疫组织化学法检测肝组织中α-SMA和E-钙黏蛋白，采用酶联免疫吸附测定法（ELISA）检测大鼠血清中ALT、AST及PCIII+PCIV+HA+LN的含量。
治疗后，模型组大鼠出现腹胀、毛发粗糙、嗜睡、精神萎靡、活动迟缓、易激惹等症状；RGJD组和秋水仙碱（Col）组的大多数大鼠精神状态良好，无明显异常活动状态。RGJD在改善肝组织形态方面显示出显著效果。RGJD组和Col组的肝纤维化分期主要分布在S1-S2期，比模型组的S3期要轻（P<0.05）。RGJD组肝脏和脾脏的湿重及指数均低于模型组（P<0.05）。此外，RGJD组E-钙黏蛋白、α-SMA的表达及AST、ALT、PCIII+PCIV的血清水平均明显低于模型组，HA和LN除外（P<0.05）。
RGJD可改善肝组织形态，降低肝纤维化血清生化指标及胶原指标，证明中药对肝纤维化的治疗效果优于Col，且能改善肝功能。
