嗜热菌产碱性α-淀粉酶的优化
OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC .
作者信息
Al-Johani Nuha Bakeet, Al-Seeni Madeha N, Ahmed Youssri Mohamed
机构信息
Biochemistry Department, Faculty of Science. King Abdulaziz University, Jeddah, Saudi Arabia.
Production of Bioproducts for Industrial Applications Research Group and Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, KSA.
出版信息
Afr J Tradit Complement Altern Med. 2016 Nov 23;14(1):288-301. doi: 10.21010/ajtcam.v14i1.31. eCollection 2017.
BACKGROUND
Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production.
MATERIALS AND METHODS
Thermophilic amylase producing bacterium was isolated from local hot water-springs in Gazan city Saudi Arabia.
RESULTS
Phylogenetic analysis of 16 S rRNA sequence for the strain revealed that the strain have the same sequence of . Maximum amylase production was observed, when cultured in medium containing starch at concentration 0.5%, and 10 g/L peptones as nitrogen source at pH 8.5 in when it was incubated for 48 h at 45°C.
CONCLUSION
An amylase-producing bacterium were isolated from hot-spring water and was identified as . Amylase produced from had optimum temperature 45°C and pH 8.5 in shaking media.
背景
淀粉降解淀粉酶在食品、发酵、纺织、造纸和制药等生物技术应用中具有重要意义。本研究旨在分离碱性耐热α-淀粉酶细菌，然后研究培养基组成和培养条件，以优化细胞生长和α-淀粉酶生产。
材料与方法
从沙特阿拉伯加赞市当地的温泉中分离出嗜热淀粉酶产生菌。
结果
对该菌株的16S rRNA序列进行系统发育分析表明，该菌株具有相同的序列。当在含有0.5%淀粉浓度的培养基中培养，以10g/L蛋白胨作为氮源，pH值为8.5，并在45°C下孵育48小时时，观察到淀粉酶产量最高。
结论
从温泉水中分离出一株淀粉酶产生菌，鉴定为 。在振荡培养基中，该菌产生的淀粉酶最适温度为45°C，最适pH值为8.5。
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