Eisen H
Blood Cells. 1978;4(1-2):177-88.
Friend virus-transformed murine erythroleukemic cells (FL cells) have been used as an in vitro model for the study of the expression of the genetic program involved in the final stages of erythroid differentiation. Treatment of the FL cells with chemical inducers such as dimethylsulfoxide results in their differentiation from 'pro-erythroblasts' to orthochromatic normoblasts and the appearance of several erythroid markers including hemoglobin, enzymes of the heme pathway, heme, glycophorin, and spectrin. These markers appear in an ordered sequence, suggesting that two genetic programs are involved in the erythroid differentiation of the cells. Preliminary studies with erythropoietin-stimulated fetal liver cultures in vitro suggest that the same is true for normal erythroid differentiation.
Friend病毒转化的小鼠红白血病细胞（FL细胞）已被用作体外模型，用于研究参与红系分化最后阶段的遗传程序的表达。用化学诱导剂如二甲基亚砜处理FL细胞，会使其从“原红细胞”分化为正成红细胞，并出现几种红系标志物，包括血红蛋白、血红素途径的酶、血红素、血型糖蛋白和血影蛋白。这些标志物按有序顺序出现，表明两个遗传程序参与了细胞的红系分化。对促红细胞生成素刺激的体外胎肝培养物的初步研究表明，正常红系分化也是如此。
