Fioritoni G, Bertolini L, Torlontano G, Revoltella R
Cancer Res. 1980 Mar;40(3):866-72.
The 3BM-78 murine Friend erythroleukemia cell line was obtained by in vitro transformation of bone marrow cells of DBA/2J mice by the polycythemic Friend virus complex. Thirty-five subclones have been isolated and tested for their ability to express various markers of blood and bone marrow cells. Upon dimethyl sulfoxide treatment, the cells differentiated along the erythroid pathway as shown by morphological evidence and by their increased synthesis of hemoglobin and spectrin. In addition, a high proportion of dimethyl sulfoxide-induced cells stained positive for specific esterase, a marker characteristic of granulocytic cells. Of these cells, about 20% stained positive for both hemoglobin-peroxidase and specific esterase. Analysis of the subclones showed that the expression of these markers for erythroid and leukopoietic differentiation was uncoordinated. Further dissection of expression was obtained by the use of two potent tumor promoters, 12-O-tetradecanoylphorbol-13-acetate and phorbol-12,13-benzoate, which in general inhibited specific esterase more than hemoglobin peroxidase expression. Inhibition was related to the structure of the phorbol diester and was unrelated to toxicity. No evidence was found for other markers characteristic of different pathways of differentiation, such as Fc and C3 receptors, cell surface immunoglobulins, theta antigen, or the capacity to phagocytose inert particles. All cells stained positive for nonspecific esterase activity in both the presence and the absence of dimethyl sulfoxide. This staining was only partially fluoride sensitive. In unstimulated cultures, a few cells also reacted for myeloperoxidase, Sudan black staining and, very rarely, alkaline phosphatase staining. These findings support the view that 3BM-78 cells are leukemic cells which, despite a prevalent commitment to erythroid differentiation, retain the genetic determinants for some traits of leukopoietic differentiation. These traits may be expressed under suitable culture conditions.
3BM - 78小鼠弗氏红白血病细胞系是通过多血症弗氏病毒复合物对DBA/2J小鼠骨髓细胞进行体外转化获得的。已分离出35个亚克隆，并检测了它们表达血液和骨髓细胞各种标志物的能力。经二甲基亚砜处理后，细胞沿红系途径分化，形态学证据以及血红蛋白和血影蛋白合成增加均表明了这一点。此外，高比例的经二甲基亚砜诱导的细胞对特异性酯酶染色呈阳性，这是粒细胞的特征性标志物。在这些细胞中，约20%对血红蛋白过氧化物酶和特异性酯酶染色均呈阳性。对亚克隆的分析表明，这些红系和白系分化标志物的表达是不协调的。通过使用两种强效肿瘤启动子，即12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯和佛波醇 - 12,13 - 二苯甲酸酯，进一步剖析了表达情况，这两种物质通常对特异性酯酶的抑制作用比对血红蛋白过氧化物酶表达的抑制作用更强。抑制作用与佛波醇二酯的结构有关，与毒性无关。未发现其他不同分化途径的特征性标志物的证据，如Fc和C3受体、细胞表面免疫球蛋白、θ抗原或吞噬惰性颗粒的能力。在有和没有二甲基亚砜的情况下，所有细胞对非特异性酯酶活性染色均呈阳性。这种染色仅部分对氟化物敏感。在未刺激的培养物中，少数细胞对髓过氧化物酶、苏丹黑染色也有反应，极少数情况下对碱性磷酸酶染色有反应。这些发现支持了这样一种观点，即3BM - 78细胞是白血病细胞，尽管它们普遍倾向于红系分化，但仍保留了白系分化某些特征的遗传决定因素。这些特征可能在合适的培养条件下表达。
