Govorkova Maria S, Milman Tatyana, Ying Gui-Shuang, Pan Wei, Silkiss Rona Z
Department of Ophthalmology, California Pacific Medical Center, San Francisco, California.
Departments of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania.
Ophthalmic Plast Reconstr Surg. 2018 May/Jun;34(3):225-230. doi: 10.1097/IOP.0000000000000921.
To evaluate the expression of inflammatory mediators in xanthelasma palpebrarum.
In this retrospective histopathologic case-control study, xanthelasma specimens obtained from the private practice and pathology archives of 1 author (R.Z.S.) were analyzed and compared with the blepharoplasty tissues from age- and sex-matched controls. Paraffin-embedded tissue sections were stained with hematoxylin-eosin and CD3, CD20, CD163, cyclooxygenase-1, inducible nitric oxide synthase, matrix metallopeptidase-9, and myeloperoxidase antibodies. Immunostaining was quantified by light microscopy and with a computerized image analysis system of scanned images.
Hematoxylin-eosin-stained preparations of xanthelasma specimens demonstrated significantly more intense chronic lymphocytic infiltrate when compared with the control blepharoplasty tissues (p < 0.001). Immunohistochemical studies revealed more intense CD3+ T cell and CD163+ histiocytic infiltrate (11% vs. 5%; p = 0.02 and 28% vs. 5%; p = 0.003, respectively) and increased expression of cyclooxygenase-1 (44% vs. 20% expressing cells; p < 0.001 and 21% vs. 9% strongly expressing cells; p = 0.008) and inducible nitric oxide synthase (43% vs. 26% expressing cells; p = 0.03 and 42% vs. 25% strongly expressing cells; p = 0.02) in xanthelasma specimens compared with control tissues.
The inflammatory milieu in xanthelasma appears to be analogous to descriptions of the early stages of cardiac atherosclerotic plaque formation. These findings may contribute to the understanding of xanthelasma pathogenesis and to the development of potential targeted therapies.
评估睑黄瘤中炎症介质的表达情况。
在这项回顾性组织病理学病例对照研究中，对从1位作者（R.Z.S.）的私人诊所和病理档案中获取的睑黄瘤标本进行分析，并与年龄和性别匹配的对照组的睑成形术组织进行比较。石蜡包埋的组织切片用苏木精-伊红以及CD3、CD20、CD163、环氧合酶-1、诱导型一氧化氮合酶、基质金属蛋白酶-9和髓过氧化物酶抗体进行染色。通过光学显微镜和扫描图像的计算机图像分析系统对免疫染色进行定量。
与对照睑成形术组织相比，睑黄瘤标本的苏木精-伊红染色制剂显示出明显更强烈的慢性淋巴细胞浸润（p < 0.001）。免疫组织化学研究显示，睑黄瘤标本中CD3⁺ T细胞和CD163⁺组织细胞浸润更强烈（分别为11%对5%；p = 0.02和28%对5%；p = 0.003），环氧合酶-1（表达细胞44%对20%；p < 0.001和强表达细胞21%对9%；p = 0.008）和诱导型一氧化氮合酶（表达细胞43%对26%；p = 0.03和强表达细胞42%对25%；p = 0.02）的表达增加。
睑黄瘤中的炎症环境似乎类似于心脏动脉粥样硬化斑块形成早期阶段的描述。这些发现可能有助于理解睑黄瘤的发病机制以及开发潜在的靶向治疗方法。
