Tsai Ching-Chung, Lee Ming-Che, Tey Shu-Leei, Liu Ching-Wen, Huang Shih-Che
Department of Pediatrics, E-Da Hospital, I-Shou University, 1, Yi-Da Road, Yan-Chao, Kaohsiung City, Taiwan, Republic of China.
School of Chinese Medicine for Post Baccalaureate, I-Shou University, 8, Yi-Da Road, Yan-Chao, Kaohsiung City, Taiwan, Republic of China.
BMC Complement Altern Med. 2017 May 8;17(1):254. doi: 10.1186/s12906-017-1752-x.
Resveratrol is a polyphenolic compound extracted from plants and is also a constituent of red wine. Resveratrol produces relaxation of vascular smooth muscle and may prevent cardiovascular diseases. Although resveratrol has been reported to cause relaxation of the guinea pig gallbladder, limited data are available about the effect of resveratrol on the gallbladder smooth muscle in humans. The purpose of this study was to investigate the relaxation effects of resveratrol in human gallbladder muscle strips.
We studied the relaxant effects of resveratrol in human gallbladder. In addition, we also investigated mechanism of resveratrol-induced relaxation in human gallbladder by tetraethylammonium (a non-selective potassium channels blocker), iberiotoxin (an inhibitor of large conductance calcium-activated potassium channel), glibenclamide (an ATP-sensitive potassium channel blocker), charybdotoxin (an inhibitor of large conductance calcium-activated potassium channels and slowly inactivating voltage-gated potassium channels), apamine (a selective inhibitor of the small conductance calcium-activated potassium channel), KT 5720 (a cAMP-dependent protein kinase A inhibitor), KT 5823 (a cGMP-dependent protein kinase G inhibitor), NG-Nitro-L-arginine (a competitive inhibitor of nitric oxide synthase), tetrodotoxin (a selective neuronal Na channel blocker), and ω-conotoxin GVIA (a selective neuronal Ca channel blocker).
The present study showed that resveratrol has relaxant effects in human gallbladder muscle strips. In addition, we found that resveratrol-induced relaxation in human gallbladder is associated with nitric oxide, ATP-sensitive potassium channel, and large conductance calcium-activated potassium channel pathways.
This study provides the first evidence concerning the relaxant effects of resveratrol in human gallbladder muscle strips. Furthermore, these results demonstrate that resveratrol is a potential new drug or health supplement in the treatment of biliary colic.
白藜芦醇是一种从植物中提取的多酚类化合物，也是红酒的成分之一。白藜芦醇可使血管平滑肌松弛，并可能预防心血管疾病。尽管已有报道称白藜芦醇可使豚鼠胆囊松弛，但关于其对人体胆囊平滑肌作用的数据有限。本研究的目的是探讨白藜芦醇对人胆囊肌条的松弛作用。
我们研究了白藜芦醇对人胆囊的松弛作用。此外，我们还通过四乙铵（一种非选择性钾通道阻滞剂）、iberiotoxin（一种大电导钙激活钾通道抑制剂）、格列本脲（一种ATP敏感性钾通道阻滞剂）、蝎毒素（一种大电导钙激活钾通道和缓慢失活电压门控钾通道抑制剂）、蜂毒明肽（一种小电导钙激活钾通道选择性抑制剂）、KT 5720（一种cAMP依赖性蛋白激酶A抑制剂）、KT 5823（一种cGMP依赖性蛋白激酶G抑制剂）、NG-硝基-L-精氨酸（一种一氧化氮合酶竞争性抑制剂）、河豚毒素（一种选择性神经元钠通道阻滞剂）和ω-芋螺毒素GVIA（一种选择性神经元钙通道阻滞剂）研究了白藜芦醇在人胆囊中诱导松弛的机制。
本研究表明白藜芦醇对人胆囊肌条有松弛作用。此外，我们发现白藜芦醇在人胆囊中诱导的松弛与一氧化氮、ATP敏感性钾通道和大电导钙激活钾通道途径有关。
本研究提供了关于白藜芦醇对人胆囊肌条松弛作用的首个证据。此外，这些结果表明白藜芦醇是治疗胆绞痛的一种潜在新药或健康补充剂。
