School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, People's Republic of China.
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, People's Republic of China.
J Pharm Sci. 2017 Aug;106(8):2053-2059. doi: 10.1016/j.xphs.2017.04.049. Epub 2017 May 5.
This research focused on optimizing the preparations of pDNA-loaded calcium phosphate (CaP) nanoparticles by employing a 3-factor, 3-level Box-Behnken design. Results indicated that a Ca/P ratio of 189.56, pH of 7.82, and a stirring speed of 528.83 rpm were the optimum conditions for preparation of the nanoparticles. The size of the optimized CaP/pDNA nanoparticles was 61.3 ± 3.64 nm, with a polydispersity index of 0.341 and an encapsulation efficiency of up to 92.11%. The optimized CaP/pDNA nanoparticles had high transfection efficiency and demonstrated good biocompatibility in vitro. Therefore, the Box-Behnken design method was successful in providing desirable CaP nanoparticle pDNA delivery systems by optimizing the experimental factors.
本研究采用三因素三水平 Box-Behnken 设计，聚焦于优化 pDNA 负载的磷酸钙（CaP）纳米粒子的制备。结果表明，Ca/P 比为 189.56、pH 值为 7.82 和搅拌速度为 528.83 rpm 是制备纳米粒子的最佳条件。优化后的 CaP/pDNA 纳米粒子的粒径为 61.3 ± 3.64nm，多分散指数为 0.341，包封效率高达 92.11%。优化后的 CaP/pDNA 纳米粒子具有较高的转染效率，在体外表现出良好的生物相容性。因此，Box-Behnken 设计方法通过优化实验因素成功提供了理想的 CaP 纳米粒子 pDNA 递药系统。
Int J Nanomedicine. 2011-12-13
Curr Drug Deliv. 2011-7
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