使用免疫层析法直接从直肠拭子和血培养瓶中检测OXA-370。
Detection of OXA-370 directly from rectal swabs and blood culture vials using an immunochromatographic assay.
作者信息
Nodari Carolina Silva, Gales Ana Cristina, Barth Afonso Luís, Magagnin Cibele Massotti, Zavascki Alexandre Prehn, Carvalhaes Cecília Godoy
机构信息
Laboratório Alerta, Division of Infectious Diseases, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, 781 Pedro de Toledo St, São Paulo, Brazil.
Laboratório Alerta, Division of Infectious Diseases, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, 781 Pedro de Toledo St, São Paulo, Brazil.
出版信息
J Microbiol Methods. 2017 Aug;139:92-94. doi: 10.1016/j.mimet.2017.05.003. Epub 2017 May 5.
We evaluated the performance of OXA-48 K-SeT assay for detecting OXA-370 directly from spiked rectal swabs and blood culture vials. The limit of detection of this test was 10UFC/mL for rectal swabs. Detection of the OXA-370-producing isolates was successfully achieved directly from positive blood culture vials independent of growing conditions.
我们评估了OXA-48 K-SeT检测法直接从加标的直肠拭子和血培养瓶中检测OXA-370的性能。该检测法对直肠拭子的检测限为10UFC/mL。直接从阳性血培养瓶中成功检测出产生OXA-370的分离株，且不受生长条件的影响。
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