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[Identification of Datura Species Involved in a Food-Poisoning Case Using LC-MS/MS and DNA Barcording].

作者信息

Ushiyama Atsuko, Akaboshi Chie, Ohsawa Nobuhiko, Shimizu Tomomi, Matsushima Yuki, Shimizu Hideaki, Hashiguchi Shigeki

机构信息

Kawasaki City Institute for Public Health.

出版信息

Shokuhin Eiseigaku Zasshi. 2017;58(2):86-95. doi: 10.3358/shokueishi.58.86.

DOI:10.3358/shokueishi.58.86
PMID:28484137
Abstract

A food-poisoning case due to eating the roots of Datura occurred in Kawasaki City, Japan in 2014. The Datura plant was mistakenly collected instead of burdock in a domestic garden. The roots of these plants are quite similar to each other. We presumed that the specimen was the root of Datura, but it was difficult to classify it only from the morphology. Using LC-MS/MS, we detected atropine and scopolamine from the remaining plant specimen. Therefore, we applied the DNA barcoding method. The results showed that the specimen was classified into Solanaceae family, but not Asteraceae family. Thus, the specimen was confirmed to be Datura species based on both chemical and genetic analyses.

摘要

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