Inaoka Takashi, Kimura Keitarou, Morimatsu Kazuya, Yamamoto Kazutaka
a Food Research Institute , National Agriculture and Food Research Organization , Tsukuba , Japan.
b Department of Food Production Science, Graduate School of Agriculture , Ehime University , Matsuyama , Japan.
Biosci Biotechnol Biochem. 2017 Jun;81(6):1235-1240. doi: 10.1080/09168451.2017.1292835. Epub 2017 Feb 22.
High hydrostatic pressure (HHP) affects various cellular processes. Using a sporulation-deficient Bacillus subtilis strain, we characterized the properties of vegetative cells subjected to HHP. When stationary-phase cells were exposed to 250 MPa of HHP for 10 min at 25 °C, approximately 50% of cells were viable, although they exhibited a prolonged growth lag. The HHP-injured cells autolyzed in the presence of NaCl or KCl (at concentrations ≥100 mM). Superoxide dismutase slightly protected the viability of HHP-treated cells, whereas vegetative catalases had no effect. Thus, unlike HHP-injured Escherichia coli, oxidative stress only slightly affected vegetative B. subtilis subjected to HHP.
高静水压（HHP）会影响多种细胞过程。我们使用一株枯草芽孢杆菌的孢子形成缺陷菌株，对经受高静水压的营养细胞的特性进行了表征。当将稳定期细胞在25℃下暴露于250兆帕的高静水压10分钟时，尽管它们表现出延长的生长延迟期，但约50%的细胞仍具有活力。高静水压损伤的细胞在存在氯化钠或氯化钾（浓度≥100毫摩尔）的情况下会自溶。超氧化物歧化酶对经高静水压处理的细胞的活力有轻微的保护作用，而营养过氧化氢酶则没有作用。因此，与受高静水压损伤的大肠杆菌不同，氧化应激对经受高静水压的枯草芽孢杆菌营养细胞的影响较小。
