Nakamura Yuta, Sato Hikari, Nobori Takanobu, Matsumoto Hotaru, Toyama Shoko, Shuno Tomohiro, Kishimura Akihiro, Mori Takeshi, Katayama Yoshiki
a Graduate School of Systems Life Sciences , Kyushu University , Fukuoka , Japan.
b Faculty of Engineering, Department of Applied Chemistry , Kyushu University , Fukuoka , Japan.
J Biomater Sci Polym Ed. 2017 Sep;28(13):1382-1393. doi: 10.1080/09205063.2017.1328730. Epub 2017 May 16.
In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.
在本研究中，我们开发了一种新技术来稳定由质粒DNA、线性聚乙烯亚胺（LPEI）和血清白蛋白组成的三元复合物。将硬脂酰基与LPEI偶联作为血清白蛋白的特异性配体。所得三元复合物在生理盐水条件下具有优异的稳定性，保持其初始直径并防止红细胞聚集。该三元复合物与未修饰的LPEI具有同等的转染能力，且细胞毒性显著更低。因此，该三元复合物作为一种具有高血液稳定性的新型基因载体具有潜在的应用价值。
