Department of Drug Supply, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Apr;21(8):1959-1966.
Postoperative cognitive dysfunction is a clinical syndrome associated with cognitive decline in patients after anesthesia. This study aimed to investigate the effect of VB12 (Vitamin B12), a kind of necessary micronutrients promoting the growth and development of the nervous system, on cognitive dysfunction induced by isoflurane anesthesia.
Eighteen-month-old rats were exposed to or were not exposed to 1.4% isoflurane for 2 h. Two hours before isoflurane exposure, rats in groups with VB12 were injected intramuscularly with VB12 at 10 or 20 μg. Two weeks later, rats were subjected to Barnes maze and Morris water maze.
Rats exposed to isoflurane had significant impairments in long-term spatial memory assessed by Barnes maze. There was no statistical significance in the percentage of swimming time and path length in the Morris water maze tests among five groups, suggesting that isoflurane may not impair the recall of learned information in rats. Isoflurane increased the expression of interleukin 1β (IL-1β) and activated caspase 3 in the hippocampus, but not cortex of the rats. The increase of IL-1β and activated caspase 3 was attenuated by VB12. However, isoflurane did not change the amount of tumor necrosis factor-α (TNF-α) and β-amyloid peptide in the hippocampus and cerebral cortex.
VB12 can attenuate cognitive dysfunction induced by isoflurane anesthesia. At the same time, IL-1β may play an important role in this isoflurane effect.
术后认知功能障碍是一种与麻醉后患者认知能力下降相关的临床综合征。本研究旨在探讨维生素 B12（Vitamin B12）对异氟醚麻醉诱导的认知功能障碍的影响。
将 18 月龄大鼠暴露于或不暴露于 1.4%异氟醚中 2 小时。在异氟醚暴露前 2 小时，给予 VB12 组大鼠肌肉注射 VB12 10 或 20 μg。2 周后，大鼠进行 Barnes 迷宫和 Morris 水迷宫测试。
暴露于异氟醚的大鼠在 Barnes 迷宫测试中表现出明显的长期空间记忆损伤。在 Morris 水迷宫测试中，五组大鼠的游泳时间百分比和路径长度没有统计学意义，提示异氟醚可能不会损害大鼠对已学习信息的回忆。异氟醚增加了大鼠海马体中白细胞介素 1β（IL-1β）的表达和半胱天冬酶 3 的激活，但对皮质无影响。VB12 可减轻 IL-1β 和激活的 caspase 3 的增加。然而，异氟醚并未改变海马体和大脑皮质中肿瘤坏死因子-α（TNF-α）和β-淀粉样肽的含量。
VB12 可减轻异氟醚麻醉诱导的认知功能障碍。同时，IL-1β 可能在这种异氟醚作用中起重要作用。
