Department of Oncology, The Affiliated Yantai Yuhuagnding Hospital of Qingdao University, Yantai, Shandong, China.
Eur Rev Med Pharmacol Sci. 2017 Apr;21(8):1746-1752.
To investigate the effect of ZEB2 silencing on cisplatin resistance in gastric cancer.
The resulting cell line, SGC7901/DDP, was transfected with ZEB2 siRNA, non-specific siRNA, or vehicle control. The effectiveness of ZEB2 silencing was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot. MTT viability assay was used to determine the cisplatin-sensitivity of cells. Cell apoptosis was measured by flow cytometry.
A significant decrease in ZEB2 in mRNA and protein level was seen in cells transfected with ZEB2 siRNA, compared to that in cells transfected with non-specific siRNA or vehicle. Transfection with ZEB2 siRNA in cisplatin-resistant SGC7901/DDP cells resulted in a significant decrease in cell viability in response to the cisplatin treatment, and cell viability decreased with increasing cisplatin concentrations. A higher apoptotic rate was also seen in cells transfected with ZEB2 siRNA under cisplatin treatment.
ZEB2 silencing can effectively make gastric cells sensitive to cisplatin treatment in vitro.
研究 ZEB2 沉默对胃癌顺铂耐药性的影响。
将 ZEB2 siRNA、非特异性 siRNA 或载体对照转染到 SGC7901/DDP 细胞系中。通过逆转录-聚合酶链反应（RT-PCR）和 Western blot 评估 ZEB2 沉默的效果。MTT 活力测定法用于测定细胞对顺铂的敏感性。通过流式细胞术测定细胞凋亡。
与转染非特异性 siRNA 或载体的细胞相比，转染 ZEB2 siRNA 的细胞中 ZEB2 在 mRNA 和蛋白水平上的表达显著降低。在顺铂耐药的 SGC7901/DDP 细胞中转染 ZEB2 siRNA 导致细胞对顺铂处理的活力显著下降，并且随着顺铂浓度的增加，细胞活力下降。在顺铂处理下，转染 ZEB2 siRNA 的细胞凋亡率也更高。
ZEB2 沉默可有效使体外胃癌细胞对顺铂治疗敏感。
