González-Sarmiento R, Greenberg J M, Kersey J H
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Blood. 1988 Dec;72(6):2038-41.
The purpose of the present study was to evaluate the use of the variable regions of the T gamma rearranging gene of the second T cell receptor. Genomic DNA was extracted from leukemic cells of T and B precursor phenotypes and hybridized to probes that detect rearrangement of the V gamma regions. We present data showing that the V gamma use is nonrandom in acute lymphoblastic leukemias (ALLs). B precursor ALL rearranged the more 5' variable regions. The results obtained suggest that the T precursor leukemias more frequently rearrange the 3' V gamma regions. Two leukemias that expressed the gamma protein rearranged V gamma 8 and V gamma 9.
本研究的目的是评估第二代T细胞受体Tγ重排基因可变区的使用情况。从T和B前体表型的白血病细胞中提取基因组DNA，并与检测Vγ区重排的探针杂交。我们提供的数据表明，Vγ的使用在急性淋巴细胞白血病（ALL）中是非随机的。B前体ALL重排了更多5'端的可变区。所得结果表明，T前体白血病更频繁地重排3'端Vγ区。两个表达γ蛋白的白血病重排了Vγ8和Vγ9。
