Instituto de Química de San Luis (INQUISAL, CCT-SanLuis), Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis 5700, Argentina; Área de Química Física, Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis 5700, Argentina.
Área de Farmacotecnia, Ética y Legislación Farmacéutica, Universidad Nacional de San Luis, Chacabuco y Pedernera, San Luis 5700, Argentina.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Sep 5;184:101-108. doi: 10.1016/j.saa.2017.04.039. Epub 2017 Apr 21.
A new green on-line method for Boldine determination (BOL) in herbal drugs and phytopharmaceuticals, using its native fluorescence in acid media (λ=282nm; λ=373nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques. Adequate dynamic calibration range (r=0.9993) was obtained over a concentration interval of 0.029-27.0μgmL BOL. The limits of detection (LOD) and quantification (LOQ) were 0.008 and 0.029μgmL, respectively. The average recoveries in explored samples ranged from 95% to 103%. Under optimized conditions, the throughput sample as high as 30h was achieved with high repeatability performance (99%). The proposed development represents a useful and valuable tool emulating the analytical efficiency of the official methodologies for quality control of herbal and phytopharmaceutical drugs containing BOL. Moreover, this approach shows advantages respect to low cost, simplicity and environmental and analyst friendly.
一种新的在线方法用于测定草药和植物药中的 Boldine（BOL），利用其在酸性介质中的本征荧光（λ=282nm；λ=373nm）。所提出的方法学首次涉及使用多壁碳纳米管作为保留剂的流动注射（FI）策略，与分子荧光相结合。通过多因素技术研究了样品 pH 和流速、洗脱液流速和组成等不同参数对 BOL 灵敏度和洗脱时间的影响。在 0.029-27.0μgmL BOL 的浓度间隔内获得了足够的动态校准范围（r=0.9993）。检测限（LOD）和定量限（LOQ）分别为 0.008 和 0.029μgmL。在探索的样品中，平均回收率在 95%到 103%之间。在优化条件下，实现了高达 30h 的高吞吐量样品，具有高重复性（99%）。该方法代表了一种有用且有价值的工具，可以模拟官方方法学的分析效率，用于控制含有 BOL 的草药和植物药的质量。此外，与低成本、简单、环境友好和对分析员友好相比，该方法具有优势。
