Perkins L M, Payne A H
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0278.
Endocrinology. 1988 Dec;123(6):2675-82. doi: 10.1210/endo-123-6-2675.
The relationship of maximal testosterone production to the amounts of cholesterol side-chain cleavage (P450scc), 17 alpha-hydroxylase/C17-20 lyase (P450(17) alpha), and iron sulfur protein (ISP) reductase was determined in Leydig cells from four inbred strains of mice (RF/J, SWR/J, C3H/He, and DBA/2). The amounts of P450scc, P450(17) alpha, and ISP reductase were also determined in adrenal glands of the same mice. cAMP-stimulated testosterone production and P450scc protein were high in RF/J and SWR/J compared to C3H/He and DBA/2 Leydig cells. A significant correlation between the amount of this enzyme and the capacity for testosterone production was found (r = 0.89; P less than 0.0005). ISP reductase was highest in RF/J, SWR/J, and C3H/He Leydig cells, which are significantly different from DBA/2. No significant differences in the amount of P450(17) alpha in Leydig cells from the four strains could be detected, and neither ISP reductase nor P450(17) alpha correlated with testosterone production. To ascertain if tissue-specific factors affect the expression of these enzymes, P450scc, ISP reductase, and P450(17) alpha were quantitated in adrenals from the same mice. P450scc and ISP reductase were expressed differently in adrenals compared to Leydig cells; levels of both proteins were high in C3H/He and RF/J adrenals compared to SWR/J and DBA/2. P450scc and ISP reductase were coordinately expressed in the adrenal, unlike in Leydig cells. P450(17) alpha was not detected in mouse adrenal glands. The results of this study suggest that strain-related differences in the capacity of Leydig cells for testosterone production may be determined by the amount of P450scc per Leydig cell. The expression of P450scc and ISP reductase in Leydig cells and adrenal glands appears to be influenced by both genetic and tissue-specific factors.
在来自四种近交系小鼠（RF/J、SWR/J、C3H/He和DBA/2）的睾丸间质细胞中，测定了最大睾酮生成量与胆固醇侧链裂解酶（P450scc）、17α-羟化酶/C17-20裂解酶（P450(17)α）以及铁硫蛋白（ISP）还原酶量之间的关系。还测定了相同小鼠肾上腺中P450scc、P450(17)α和ISP还原酶的量。与C3H/He和DBA/2睾丸间质细胞相比，RF/J和SWR/J中cAMP刺激的睾酮生成及P450scc蛋白含量较高。发现该酶的量与睾酮生成能力之间存在显著相关性（r = 0.89；P < 0.0005）。RF/J、SWR/J和C3H/He睾丸间质细胞中的ISP还原酶含量最高，与DBA/2有显著差异。在来自这四个品系的睾丸间质细胞中，未检测到P450(17)α量的显著差异，且ISP还原酶和P450(17)α均与睾酮生成无关。为确定组织特异性因素是否影响这些酶的表达，对相同小鼠肾上腺中的P450scc、ISP还原酶和P450(17)α进行了定量。与睾丸间质细胞相比，肾上腺中P450scc和ISP还原酶的表达不同；与SWR/J和DBA/2相比，C3H/He和RF/J肾上腺中这两种蛋白的水平较高。与睾丸间质细胞不同，P450scc和ISP还原酶在肾上腺中协同表达。在小鼠肾上腺中未检测到P450(17)α。本研究结果表明，睾丸间质细胞睾酮生成能力的品系相关差异可能由每个睾丸间质细胞中P450scc的量决定。P450scc和ISP还原酶在睾丸间质细胞和肾上腺中的表达似乎受遗传和组织特异性因素的影响。
