Unité de recherche sur les maladies infectieuses et tropicale émergentes (URMITE), UM 63 CNRS 7278 IRD 198 INSERM U1905, IHU Méditerranée Infection, Facultés de médecine et de pharmacie, Marseille, France; Laboratoire de microbiologie, Département de biochimie, Université Badji-Mokhtar, Annaba, Algeria.
Unité de recherche sur les maladies infectieuses et tropicale émergentes (URMITE), UM 63 CNRS 7278 IRD 198 INSERM U1905, IHU Méditerranée Infection, Facultés de médecine et de pharmacie, Marseille, France; Laboratoire de biotechnologie des molécules bioactives et de la physiopathologie cellulaire (LBMBPC), Faculté des sciences de la nature et de la vie, Université de Batna 2, Fesdis, Algeria.
J Glob Antimicrob Resist. 2017 Jun;9:103-110. doi: 10.1016/j.jgar.2017.02.010. Epub 2017 May 6.
The main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons.
Stool samples were collected from 73 pigeons in two Mediterranean cities, namely Marseille (France) and Annaba (Algeria). Faecal samples were screened by real-time PCR and standard PCR for the presence of carbapenemase-encoding genes.
Carbapenem resistance genes were detected in 16 (21.9%) of the samples, with 8 positive for bla, 12 positive for bla and 13 positive for bla. No samples were positive for bla, bla, bla, bla or bla. All positive samples were screened for the presence of Acinetobacter spp. by partial rpoB gene sequence amplification, and the results showed the presence of five Acinetobacter spp., with percentage similarities to related species in GenBank ranging between 96% and 100%. The dominant species was Acinetobacter guillouiae, followed by Acinetobacter baumannii, Acinetobacter haemolyticus, Acinetobacter pittii and Acinetobacter nosocomialis. One DNA sequence showed a very low degree of homology (92%) with Acinetobacter gerneri, suggesting a new Acinetobacter spp.
Here we report the first detection of carbapenemase-encoding genes from urban pigeon stools. These results question the potential of birds as a reservoir for the spread of these resistance determinants both in animals and humans.
本研究的主要目的是检测城市鸽子粪便样本中是否存在碳青霉烯酶编码基因。
从法国马赛和阿尔及利亚安纳巴这两个地中海城市的 73 只鸽子中采集粪便样本。通过实时 PCR 和标准 PCR 检测粪便样本中是否存在碳青霉烯酶编码基因。
在 16 份（21.9%）样本中检测到碳青霉烯类耐药基因，bla 阳性 8 份，bla 阳性 12 份，bla 阳性 13 份。bla、bla、bla、bla 或 bla 基因均未检出。所有阳性样本均通过 rpoB 基因部分序列扩增筛选是否存在不动杆菌属，结果显示有 5 种不动杆菌属，与 GenBank 中相关种的相似性百分比在 96%至 100%之间。优势种为鲁氏不动杆菌，其次为鲍曼不动杆菌、溶血不动杆菌、派氏不动杆菌和洛菲不动杆菌。有一个 DNA 序列与阿氏不动杆菌的同源性非常低（92%），提示可能是一种新的不动杆菌属。
本研究首次从城市鸽子粪便中检测到碳青霉烯酶编码基因。这些结果表明鸟类可能是这些耐药决定因素在动物和人类中传播的潜在储主。
