Yan Xingke, Zhang Ao, Yu Lu, Chen Cheng, Cui Haifu
College of Acupuncture, Moxibustion and Tuina, Gansu University of Chinese Medicine, Lanzhou 730000, China.
Jilin Provincial Jilin Hospital of Integrated Traditional Chinese and Western Medicine, Jilin 132021, China.
Can Respir J. 2017;2017:8015703. doi: 10.1155/2017/8015703. Epub 2017 Apr 11.
. To investigate the pathogenesis of allergic asthma via the nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique. . 24 Wistar rats were randomized into 3 groups: the blank-control group (A), the restrained group (B), and the asthma model group (C). The nanoliter two-dimensional liquid chromatography (nano-2D-LC) technique was used to study the differential protein expressions of the serum in asthmatic rats. . Compared with the restrained group, the data from the asthma model group displayed a distinctive peak, that is, peak number 13 (94.731 min). The data also displayed three missing peaks in the asthma group, that is, peak number 1 (77.489 min), peak number 2 (78.418 min), and peak number 5 (80.533 min), suggesting that the corresponding peptides might be related to the pathogenesis of asthma. Compared with the blank-control group, the restrained group showed 4 new peaks, that is, peak number 2 (78.418 min), peak number 4 (79.398 min), peak number 5 (80.533 min), and peak number 7 (81.824 min). The restrained group also displayed a missing peak, that is, peak number 3 (78.804 min), indicating that those 5 polypeptides might be related to the binding-induced stress stimuli. . The study suggests that the pathogenesis of allergic asthma is closely related to abnormal levels of proteins; however, future animal experiments should identify the specific protein expressions caused by stress factors.
通过纳升二维液相色谱（nano-2D-LC）技术研究过敏性哮喘的发病机制。将24只Wistar大鼠随机分为3组：空白对照组（A组）、束缚组（B组）和哮喘模型组（C组）。采用纳升二维液相色谱（nano-2D-LC）技术研究哮喘大鼠血清中蛋白质的差异表达。与束缚组相比，哮喘模型组的数据显示出一个独特的峰，即峰13（94.731分钟）。数据还显示哮喘组有三个缺失峰，即峰1（77.489分钟）、峰2（78.418分钟）和峰5（80.533分钟），表明相应的肽可能与哮喘的发病机制有关。与空白对照组相比，束缚组显示出4个新峰，即峰2（78.418分钟）、峰4（79.398分钟）、峰5（80.533分钟）和峰7（81.824分钟）。束缚组还显示出一个缺失峰，即峰3（78.804分钟），表明这5种多肽可能与束缚诱导的应激刺激有关。该研究表明，过敏性哮喘的发病机制与蛋白质水平异常密切相关；然而，未来的动物实验应确定应激因素引起的具体蛋白质表达。
