Gallez B, Osinski P, Adline J, Dumont P
Catholic University of Louvain, School of Pharmacy, Department of Medicinal Chemistry, Brussels, Belgium.
Int J Rad Appl Instrum A. 1988;39(9):1011-4. doi: 10.1016/0883-2889(88)90047-0.
Two parallel, independent micro-methods for diphosphonate determination are presented. These procedures are based on the interference of diphosphonate with the formation of cation-morin (3,5,7,2',4'-pentahydroxyflavone) complexes. In the spectrophotometric method, a change of the absorbance of thorium-morin complex is used as a measure of diphosphonate concentration. In the fluorimetric method, a decrease of the fluorescence of aluminium-morin complex is used. Both methods: (1) quantitate methylene-diphosphonate in the range of 5-40 x 10(-9) M with a coefficient of variation of less than 1%; (2) are specific and interference-free in commercial kits control; (3) are operative with other diphosphonates; and (4) are adaptable to the analysis of chromatographic eluents.
本文介绍了两种平行、独立的双膦酸盐测定微方法。这些方法基于双膦酸盐对阳离子-桑色素（3,5,7,2',4'-五羟基黄酮）络合物形成的干扰。在分光光度法中，钍-桑色素络合物吸光度的变化被用作双膦酸盐浓度的度量。在荧光法中，铝-桑色素络合物荧光的降低被采用。两种方法：（1）在5 - 40×10⁻⁹ M范围内对亚甲基双膦酸盐进行定量，变异系数小于1%；（2）在商业试剂盒对照中具有特异性且无干扰；（3）对其他双膦酸盐也适用；（4）适用于色谱洗脱液的分析。
