Lee Hyunsu, Cho Ji-Hyoung, Park Won-Jin, Jung Soo-Jung, Choi In-Jang, Lee Jae-Ho
Department of Anatomy, School of Medicine, Keimyung University, 2800, Dalgubeoldaero, Dalseo-Gu, Daegu, Republic of Korea.
Department of General Surgery, School of Medicine, Keimyung University, Daegu, South Korea.
Pathol Oncol Res. 2018 Apr;24(2):323-328. doi: 10.1007/s12253-017-0245-z. Epub 2017 May 9.
Positive association between telomere length and mitochondrial DNA (mtDNA) copy number were introduced in healthy and patients with psychiatric disorder. Based on frequent genetic changes of telomere and mitochondria in colorectal carcinomas (CRC), we studied their clinical characteristics and their association in colorectal carcinogenesis. DNA was extracted from 109 CRCs, 64 colorectal tubular adenomas (TAs), and 28 serrated polyps (SPs), and then, telomere length and mtDNA copy number were analyzed in these legions by using a real-time PCR assay. Telomere length and mtDNA copy number (mean ± S.D) in CRCs was 1.87 ± 1.52 and 1.61 ± 1.37, respectively. In TAs and SPs, relative mtDNA copy number was 0.92 ± 0.71 and 1.84 ± 1.06, respectively, shoing statistical difference (p = 0.017). However, telomere length was similar in these precancerous legions. Telomere length and mtDNA copy number did not show clinical and prognostic values in CRCs, however, positive correlation between telomere length and mitochondrial DNA copy number were found in CRC (r = 0.408, p < 0.001). However, this association was not shown in precancerous lesions (r = -0.031, p = 0.765). This result suggests that loss of co-regulation between telomeres and mitochondrial function may induce the initiation or play a role as trigger factor of colorectal carcinogenesis.
端粒长度与线粒体DNA（mtDNA）拷贝数之间的正相关关系在健康人群和精神疾病患者中已有报道。基于结直肠癌（CRC）中端粒和线粒体频繁的基因变化，我们研究了它们在结直肠癌发生过程中的临床特征及其关联。从109例CRC、64例大肠管状腺瘤（TA）和28例锯齿状息肉（SP）中提取DNA，然后使用实时PCR测定法分析这些病变中的端粒长度和mtDNA拷贝数。CRC中的端粒长度和mtDNA拷贝数（平均值±标准差）分别为1.87±1.52和1.61±1.37。在TA和SP中，相对mtDNA拷贝数分别为0.92±0.71和1.84±1.06，显示出统计学差异（p = 0.017）。然而，这些癌前病变中的端粒长度相似。端粒长度和mtDNA拷贝数在CRC中未显示出临床和预后价值，然而，在CRC中发现端粒长度与线粒体DNA拷贝数之间存在正相关（r = 0.408，p < 0.001）。然而，这种关联在癌前病变中未显示（r = -0.031，p = 0.765）。这一结果表明，端粒与线粒体功能之间协同调节的丧失可能诱导结直肠癌的发生或作为触发因素发挥作用。
