用同种异体原位成纤维细胞或骨髓来源的间充质基质细胞重新定植的脱细胞髂胫束

Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells.

作者信息

Gögele Clemens, Schwarz Silke, Ondruschka Benjamin, Hammer Niels, Schulze-Tanzil Gundula

机构信息

Institute of Anatomy, Paracelsus Medical University, Salzburg and Nuremberg, Prof.-Ernst-Nathan Str. 1, Nuremberg, 90419, Germany.

Institute of Legal Medicine, University of Leipzig, Johannisallee 28, Leipzig, 04103, Germany.

出版信息

Methods Mol Biol. 2018;1577:55-69. doi: 10.1007/7651_2017_30.

DOI:10.1007/7651_2017_30

PMID:28488244

Abstract

Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs).

摘要

去细胞支架在组织工程的各个领域展现出了有前景的仿生方法。不同组织已被选用于去细胞处理，其中包括富含细胞外基质（ECM）的组织，如肌腱、韧带和软骨。韧带致密的细胞外基质要实现完全无细胞且无免疫原性极具挑战性。在此，介绍了适用于髂胫束（ITB）韧带组织的去细胞方法，以及使用同种异体ITB来源的成纤维细胞或间充质基质细胞（MSC）进行细胞分离和几种再定植技术。