BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada.
Sensors (Basel). 2017 May 10;17(5):1079. doi: 10.3390/s17051079.
Enzyme-linked immunosorbent assay (ELISA) is a popular assay technique for the detection and quantification of various biological substances due its high sensitivity and specificity. More often, it requires large and expensive laboratory instruments, which makes it difficult to conduct when the tests must be performed quickly at the point-of-care (POC). To increase portability and ease of use, we propose a portable diagnostic system based on a Raspberry Pi imaging sensor for the rapid detection of progesterone in milk samples. We designed, assembled, and tested a standalone portable diagnostic reader and validated it for progesterone detection against a standard ELISA assay using a commercial plate reader. The portable POC device yielded consistent results, regardless of differences in the cameras and flashlights between various smartphone devices. An Android application was built to provide front-end access to users, control the diagnostic reader, and display and store the progesterone measurement on the smartphone. The diagnostic reader takes images of the samples, reads the pixel values, processes the results, and presents the results on the handheld device. The proposed POC reader can perform to superior levels of performance as a plate reader, while adding the desirable qualities of portability and ease of use.
酶联免疫吸附测定（ELISA）是一种广泛应用于检测和定量各种生物物质的分析技术，因为其具有高灵敏度和特异性。然而，它通常需要大型和昂贵的实验室仪器，这使得在需要快速进行床边即时检测（POC）时难以实施。为了提高便携性和易用性，我们提出了一种基于 Raspberry Pi 成像传感器的便携式诊断系统，用于快速检测牛奶样本中的孕酮。我们设计、组装和测试了一个独立的便携式诊断读取器，并使用商业平板读取器对其进行了孕酮检测验证，以标准 ELISA 分析作为对照。该便携式即时检测设备产生了一致的结果，而不受各种智能手机设备之间的相机和闪光灯差异的影响。我们还构建了一个 Android 应用程序，为用户提供前端访问、控制诊断读取器，并在智能手机上显示和存储孕酮测量值。诊断读取器拍摄样本图像、读取像素值、处理结果，并在手持设备上呈现结果。与平板读取器相比，所提出的即时检测读取器具有更高的性能水平，同时还具有便携性和易用性等理想品质。
