Thiel R, Schreurs W J, Harold F M
Department of Molecular and Cellular Biology, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
J Gen Microbiol. 1988 May;134(5):1089-97. doi: 10.1099/00221287-134-5-1089.
Changes in the pattern of electric currents that accompany the transformation of growing hyphae of Achlya bisexualis into sporangia have been examined. When hyphae were transferred to a non-nutrient buffer, they continued to extend for several hours and then gave rise to sporangia. Throughout this process, current (positive charge) flowed into the apical region that corresponds approximately to the future sporangium. The current ceased after the crosswall appeared. The sporangium then remained electrically quiescent, except for a brief intense burst of outward current at the 'homogeneous' stage of spore cleavage. The inward current during sporangium formation largely represents an influx of protons. Addition of nitrate abolished the flow of electric current with little effect on sporulation. The late burst of outward current is most probably an artefact, generated by the discharge of salts from the sporangial vacuole. The transcellular electric current apparently plays no role in sporangium formation or in spore cleavage. Calcium ions, however, are required and may traverse the plasma membrane.
已经研究了双性绵霉生长菌丝转变为孢子囊过程中伴随的电流模式变化。当菌丝转移到无营养缓冲液中时，它们会继续延伸数小时，然后形成孢子囊。在整个过程中，电流（正电荷）流入大致对应于未来孢子囊的顶端区域。横壁出现后电流停止。然后孢子囊保持电静止状态，除了在孢子分裂的“均匀”阶段有短暂的强烈外向电流爆发。孢子囊形成过程中的内向电流主要代表质子的流入。添加硝酸盐消除了电流流动，对孢子形成影响很小。外向电流的后期爆发很可能是一种假象，由孢子囊液泡中的盐分释放产生。跨细胞电流显然在孢子囊形成或孢子分裂中不起作用。然而，钙离子是必需的，并且可能穿过质膜。
