介质阻挡放电（DBD）等离子体对大豆胰蛋白酶抑制剂的灭活作用。
Inactivation of soybean trypsin inhibitor by dielectric-barrier discharge (DBD) plasma.
作者信息
Li Junguang, Xiang Qisen, Liu Xiufang, Ding Tian, Zhang Xiangsheng, Zhai Yafei, Bai Yanhong
机构信息
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Henan Collaborative Innovation Center of Food Production and Safety, Zhengzhou 450001, PR China.
College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Henan Collaborative Innovation Center of Food Production and Safety, Zhengzhou 450001, PR China.
出版信息
Food Chem. 2017 Oct 1;232:515-522. doi: 10.1016/j.foodchem.2017.03.167. Epub 2017 Apr 6.
Soybean trypsin inhibitor (STI) is considered as one of the most important anti-nutritional factors in soybeans. The objective of this study was to investigate the impacts and underling mechanisms of dielectric-barrier discharge (DBD) plasma on STI activities. The results shown that DBD plasma treatment significantly induced the inactivation of STI in soymilk and Kunitz-type trypsin inhibitor from soybean (SKTI) in a model system. After exposure to DBD plasma at 51.4W for 21min, the STI activities of soymilk were reduced by 86.1%. Affter being treated by DBD plasma, the intrinsic fluorescence and surface hydrophobicity of SKTI were significantly decreased, while the sulfhydryl contents were increased. It is assumed that DBD plasma-induced conformational changes and oxidative modification might contribute to the inactivation of SKTI. In summary, DBD plasma technology is a potential alternative to heat treatment for the inactivation of anti-nutritional substances in food legumes.
大豆胰蛋白酶抑制剂（STI）被认为是大豆中最重要的抗营养因子之一。本研究的目的是探讨介质阻挡放电（DBD）等离子体对STI活性的影响及其潜在机制。结果表明，DBD等离子体处理显著诱导了豆乳中STI以及模型体系中大豆Kunitz型胰蛋白酶抑制剂（SKTI）的失活。在51.4W的DBD等离子体下暴露21分钟后，豆乳中STI的活性降低了86.1%。经DBD等离子体处理后，SKTI的固有荧光和表面疏水性显著降低，而巯基含量增加。推测DBD等离子体诱导的构象变化和氧化修饰可能导致SKTI失活。综上所述，DBD等离子体技术是豆类食品中抗营养物质失活热处理的一种潜在替代方法。
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