Li Huibin, Chen Fang
Department of Burn and Plastic Surgery, People's Hospital of Linyi, Linyi, Shandong, PR China.
Department of Orthopedics, People's Hospital of Linyi, Linyi, Shandong, PR China.
Saudi J Biol Sci. 2017 May;24(4):797-802. doi: 10.1016/j.sjbs.2015.10.025. Epub 2015 Nov 10.
The aim of this work was to prepare coenzyme Q10 (CoQ10) long-circulating liposomes, and establish the quality standard to determine the content and entrapment efficiency. CoQ10 long-circulating liposomes were prepared by the film dispersion method, HPLC assay for the determination of CoQ10 was developed. Free drugs and liposomes were separated using the protamine aggregation method and entrapment efficiency was determined. The liposomes were homogeneous and the mean diameter was 166.0 nm, Zeta potential was -22.2 mV. The content and entrapment efficiency of CoQ10 were 98.2% and 93.2% for three batches of liposomes, respectively. The lyophilized form of liposomes prepared by freeze-drying showed stable quality characteristics during storage. The formulation and preparative method can be used to prepare CoQ10 long-circulating liposomes with high entrapment efficiency and high quality, the determination method of drug content and entrapment efficiency were effective and rapid and can be used for quality evaluation of liposomes.
本研究旨在制备辅酶Q10（CoQ10）长循环脂质体，并建立其含量及包封率的质量控制标准。采用薄膜分散法制备CoQ10长循环脂质体，建立了HPLC法测定CoQ10的含量。采用鱼精蛋白凝聚法分离游离药物与脂质体，测定包封率。制备的脂质体形态均匀，平均粒径为166.0 nm，Zeta电位为-22.2 mV。三批脂质体中CoQ10的含量及包封率分别为98.2%和93.2%。冻干法制得的脂质体冻干粉在储存过程中质量特性稳定。该制剂及制备方法可用于制备包封率高、质量好的CoQ10长循环脂质体，所建立的药物含量及包封率测定方法准确、快速，可用于脂质体的质量评价。
