Bich G A, Castrillo M L, Villalba L L, Zapata P D
Microbiology and Immunology Laboratory, Misiones National University, 1375, Mariano Moreno Ave., 3300 Posadas, Misiones, Argentina.
Biotechnology Institute of Misiones "María Ebe Reca", 12 Road, km 7, 5, 3300 Misiones, Argentina.
Saudi J Biol Sci. 2017 May;24(4):851-856. doi: 10.1016/j.sjbs.2016.05.010. Epub 2016 May 19.
Leaf-cutting ants live in an obligate symbiosis with a species, a basidiomycete that serves as a food source to the larvae and queen. The aim of this work was to isolate, identify and complete the phylogenetic study of species of . Macroscopic and microscopic features were used to identify the fungal symbiont of the ants. The ITS1-5.8S-ITS2 region was used as molecular marker for the molecular identification and to evaluate the phylogeny within the genus. One fungal symbiont associated with was isolated and identified as . The phylogeny of obtained using the ITS molecular marker revealed three well established monophyletic groups. It was possible to recognize one clade of associated with phylogenetically derived leaf-cutting ants ( and ). A second clade of free living forms of (non-cultivated), and a third clade of associated with phylogenetically basal genera of ants were also recognized. The clades corresponded to traditional taxonomic groups, and were differentiated by ecological habitats of different species.
切叶蚁与一种担子菌存在专性共生关系，这种担子菌是幼虫和蚁后的食物来源。这项工作的目的是分离、鉴定并完成对该担子菌物种的系统发育研究。利用宏观和微观特征来鉴定蚂蚁的真菌共生体。ITS1-5.8S-ITS2区域被用作分子标记进行分子鉴定，并评估该担子菌属内的系统发育。分离出一种与切叶蚁相关的真菌共生体，并鉴定为该担子菌。使用ITS分子标记获得的该担子菌的系统发育显示出三个成熟的单系群。可以识别出一个与系统发育衍生的切叶蚁（Atta和Acromyrmex）相关的担子菌分支。还识别出第二个自由生活形式（非培养）的担子菌分支，以及第三个与系统发育基部蚂蚁属相关的担子菌分支。这些分支对应于传统分类群，并因不同物种的生态栖息地而有所区别。
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