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依赖于NifA的体内保护作用表明，固氮酶基因启动子的上游激活序列是一个蛋白质结合位点。

NifA-dependent in vivo protection demonstrates that the upstream activator sequence of nif promoters is a protein binding site.

作者信息

Morett E, Buck M

机构信息

Agricultural & Food Research Council, University of Sussex, Brighton, United Kingdom.

出版信息

Proc Natl Acad Sci U S A. 1988 Dec;85(24):9401-5. doi: 10.1073/pnas.85.24.9401.

DOI:10.1073/pnas.85.24.9401
PMID:2849102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282760/
Abstract

Primer-extension analysis of the Klebsiella pneumoniae nifH promoter was used to determine changes in the accessibility of the promoter DNA to methylation after exposure of growing cells to dimethyl sulfate. Four guanine residues present in the nifH upstream activator sequence (UAS), the proposed NifA binding site, were protected from methylation and two guanine residues were hypermethylated when the transcriptional activator protein NifA was present in the cells. The interaction detected at the nifH UAS was independent of the alternative sigma factor NtrA required for transcription of the nifH and other nif promoters. Mutations within the nifH UAS that diminish NifA-dependent transcriptional activation reduced the interaction at the UAS. It seems likely that the pattern of methylation protection observed in the nifH UAS is the result of NifA binding.

摘要

利用肺炎克雷伯菌nifH启动子的引物延伸分析来确定生长中的细胞暴露于硫酸二甲酯后启动子DNA甲基化可及性的变化。当细胞中存在转录激活蛋白NifA时，nifH上游激活序列（UAS，即推测的NifA结合位点）中存在的四个鸟嘌呤残基受到甲基化保护，而两个鸟嘌呤残基发生超甲基化。在nifH UAS处检测到的相互作用独立于nifH和其他nif启动子转录所需的替代σ因子NtrA。nifH UAS内降低NifA依赖性转录激活的突变减少了在UAS处的相互作用。nifH UAS中观察到的甲基化保护模式似乎是NifA结合的结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/51ca358d465e/pnas00303-0040-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/9a353d01e0c7/pnas00303-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/3fb745d34ad2/pnas00303-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/0c3b3a2c6739/pnas00303-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/afbf13449eb0/pnas00303-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/51ca358d465e/pnas00303-0040-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/9a353d01e0c7/pnas00303-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/3fb745d34ad2/pnas00303-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/0c3b3a2c6739/pnas00303-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/afbf13449eb0/pnas00303-0040-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccbb/282760/51ca358d465e/pnas00303-0040-c.jpg

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Mutations affecting regulation of the Klebsiella pneumoniae nifH (nitrogenase reductase) promotor.影响肺炎克雷伯菌nifH（固氮酶还原酶）启动子调控的突变
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The products of glnL and glnG are bifunctional regulatory proteins.glnL和glnG的产物是双功能调节蛋白。
CowN sustains nitrogenase turnover in the presence of the inhibitor carbon monoxide.
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J Biol Chem. 2021 Jan-Jun;296:100501. doi: 10.1016/j.jbc.2021.100501. Epub 2021 Mar 2.
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NifA is the master regulator of both nitrogenase systems in Rhodobacter capsulatus.NifA 是荚膜红细菌中两种氮酶系统的主要调节因子。
Microbiologyopen. 2019 Dec;8(12):e921. doi: 10.1002/mbo3.921. Epub 2019 Aug 22.
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structural homology modeling of nif A protein of rhizobial strains in selective legume plants.选择性豆科植物中根瘤菌菌株固氮调节蛋白A的结构同源性建模
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