[Studies on aldosterone RIA kit using the two antibody method].

Two antibody method of aldosterone RIA kit was investigated. This kit was measured directly without extracting the aldosterone. On fundamental studies, stability of standard curve, reproductivity, recovery test and dilution test were obtained good results. On clinical studies, the diurnal variation of serum aldosterone concentration in normal child and adult was correlated well to that of concentration of cortisol and ACTH. The concentration of three hormones was the highest in the early morning and it was the lowest in midnight.