Martins Thiago F, Luz Hermes R, Faccini João Luiz H, Labruna Marcelo B
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.
Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, UFRRJ, Seropédica, Rio de Janeiro, Brazil.
Exp Appl Acarol. 2017 Apr;71(4):415-424. doi: 10.1007/s10493-017-0135-9. Epub 2017 May 10.
This study evaluated for the first time the life cycle of Amblyomma oblongoguttatum in the laboratory. For this purpose, larvae and nymphs were exposed to Gallus gallus (chicks), Rattus norvegicus (wistar rat), Calomys callosus (vesper mouse), Oryctolagus cuniculus (domestic rabbit), Cavia porcellus (guinea pig), and Didelphis albiventris (white-eared opossum). Nymphs were exposed to G. gallus, C. callosus, C. porcellus, O. cuniculus, R. norvegicus, and Nectomys squamipes (water rat). Adult ticks were exposed to domestic dogs. The life-cycle of A. oblongoguttatum in the laboratory could be completed in an average period of 188 days, considering prefeeding periods of 25 days for each of the parasitic stages. Under laboratory conditions, none of the host species was highly suitable for A. oblongoguttatum larvae, since the recovery rates of engorged larvae were always <15%, or most of the times ≤5%. Similar results were obtained for nymphs, with recovery rates of engorged nymphs always <6%. Our results, coupled with literature data, suggest that small mammals, especially small rodents, do not have an important role in the life-cycle of A. oblongoguttatum under field conditions. Domestic dogs showed to be highly suitable for the adult stage of A. oblongoguttatum, in agreement with literature data that have appointed dogs as important hosts for the adult stage of A. oblongoguttatum in South America.
本研究首次在实验室评估了长盾璃眼蜱的生命周期。为此，将幼虫和若虫暴露于原鸡（雏鸡）、褐家鼠（Wistar大鼠）、卡氏棉鼠（ Vesper小鼠）、穴兔（家兔）、豚鼠、和白腹袋鼩（白耳负鼠）。将若虫暴露于原鸡、卡氏棉鼠、豚鼠、穴兔、褐家鼠和鳞尾木鼠（水鼠）。将成年蜱暴露于家犬。考虑到每个寄生阶段25天的预饲期，长盾璃眼蜱在实验室的生命周期平均可在188天内完成。在实验室条件下，没有一种宿主物种对长盾璃眼蜱幼虫非常适宜，因为饱血幼虫的回收率始终<15%，大多数时候≤5%。若虫也得到了类似的结果，饱血若虫的回收率始终<6%。我们的结果与文献数据表明，在野外条件下，小型哺乳动物，尤其是小型啮齿动物，在长盾璃眼蜱的生命周期中没有重要作用。家犬被证明对长盾璃眼蜱的成虫阶段非常适宜，这与将犬类指定为南美洲长盾璃眼蜱成虫阶段重要宿主的文献数据一致。
