Department of Polymer Science & Engineering, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210023, P. R. China.
ACS Appl Mater Interfaces. 2017 May 24;9(20):16735-16740. doi: 10.1021/acsami.7b01987. Epub 2017 May 15.
There is urgent demand of easily available and highly effective method to improve transgene performance of polymeric gene carriers at low consumption of delivery materials. We developed biocompatible multicomponent nanocomposites in which small quantities of cationic polythiophenes were engineered into the outer shell of polypeptide/DNA polyplexes without covalent linkages. We revealed the introduction of polythiophenes in small quantities led to multiple outcomes including modulation of polyplex size and zeta potential, increase in polyplex stability, promotion of endolysosome membrane disruption, light-induced generation of reactive oxygen species (ROS), and significant enhancement of gene delivery to tumor cells. The factors such as structural architectures, molecular weights, photosensitizing capability, and percentage composition of polythiophenes were investigated.
目前非常需要一种简便且高效的方法，以在低用量的给药材料下，改善聚合物基因载体的转基因性能。我们开发了生物相容性的多组分纳米复合材料，其中将少量的阳离子聚噻吩通过非共价键设计到多肽/DNA 超分子复合物的外壳中。我们揭示了小剂量的聚噻吩的引入会导致多种结果，包括调节超分子复合物的大小和 Zeta 电位、增加超分子复合物的稳定性、促进内溶酶体膜的破坏、光诱导产生活性氧（ROS），以及显著提高基因向肿瘤细胞的传递。我们研究了聚噻吩的结构架构、分子量、光敏能力和百分组成等因素。
