Facultad de Medicina, Universidad de las Américas, Quito, Ecuador.
Pharmaceutical Science Division, University of Wisconsin, Madison, WI, USA.
Int Microbiol. 2016 Sep;19(3):157-160. doi: 10.2436/20.1501.01.273.
Enteropathogenic Escherichia coli (EPEC) remain one the most important pathogens infecting children and they are one of the main causes of persistent diarrhea worldwide. In this study, we have isolated EPEC from 94 stool samples of children under five years old with diarrheal illness in the area of Quito (Ecuador), and we have determined the occurrence of the two subtypes of EPEC, typical EPEC (tEPEC) and atypical (aEPEC), by PCR amplification of the genes eae (attaching and effacing) and bfp (bundle- forming pilus). Typical EPEC is positive for eae and bfp genes while aEPEC is positive only for eae. Our results suggest that aEPEC is the most prevalent subtype in Quito (89.36 %), while subtype tEPEC is less prevalent (10.64 %). [Int Microbiol 19(3):157-160 (2016)].
肠致病性大肠杆菌（EPEC）仍然是感染儿童的最重要病原体之一，也是全球持续性腹泻的主要原因之一。在这项研究中，我们从基多（厄瓜多尔）地区 94 份五岁以下腹泻儿童的粪便样本中分离出了 EPEC，并通过 PCR 扩增 eae（黏附与破坏）和 bfp（束形成菌毛）基因来确定 EPEC 的两种亚型，即典型 EPEC（tEPEC）和非典型（aEPEC）。典型 EPEC 对 eae 和 bfp 基因均呈阳性，而非典型 EPEC 仅对 eae 基因呈阳性。我们的结果表明，在基多，aEPEC 是最常见的亚型（89.36%），而亚型 tEPEC 则较少见（10.64%）。[Int Microbiol 19(3):157-160 (2016)]。
