Wu Lin, Du Zhong-Rui, Xu Ai-Li, Yan Zhen, Xiao Hui-Hui, Wong Man-Sau, Yao Xin-Sheng, Chen Wen-Fang
Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines, Medical College of Qingdao University, Qingdao 266071, PR China; Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, PR China; Department of Physiology, Heze Medical College, Heze 274000, PR China.
Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines, Medical College of Qingdao University, Qingdao 266071, PR China; Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, PR China.
Biomed Pharmacother. 2017 Jul;91:656-663. doi: 10.1016/j.biopha.2017.04.083. Epub 2017 May 8.
Flavonoids, the active components of Epimedii Genus, have been demonstrated to protect against osteoporosis, cardiovascular diseases and rheumatoid arthritis. The present study aimed to investigate the neuroprotective effects of total flavonoid (TF) fraction of Epimedium koreanum Nakai on dopaminergic neurons in the cellular and mice models of Parkinson's disease (PD). TF pretreatment could ameliorate the decrease of striatal dopamine (DA) content and the loss of tyrosine hydroxylase (TH)-immunoreactive neurons in the substantia nigra pars compacta (SNpc) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). TF treatment could reverse the changes of Bcl-2 and Bax protein expressions in the striatum of PD mice. 1-Methyl-4-phenylpyridinium ion (MPP) significantly decreased the cell viability and mitochondrial membrane potential in MES23.5 cells. These effects could be reversed by TF treatment. In addition, MPP-induced changes of Bcl-2 and Bax mRNA and protein expressions were also reversed by TF pretreatment. These data demonstrated that TF of E. koreanum Nakai could protect against MPTP-induced dopaminergic neuronal death in mice and MPP-induced neurotoxicity in dopaminergic MES23.5 cells. Anti-apoptosis might be involved in this process.
黄酮类化合物是淫羊藿属植物的活性成分，已被证明具有预防骨质疏松症、心血管疾病和类风湿性关节炎的作用。本研究旨在探讨朝鲜淫羊藿总黄酮（TF）组分在帕金森病（PD）细胞模型和小鼠模型中对多巴胺能神经元的神经保护作用。TF预处理可改善1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的纹状体多巴胺（DA）含量降低以及黑质致密部（SNpc）中酪氨酸羟化酶（TH）免疫反应性神经元的丢失。TF治疗可逆转PD小鼠纹状体中Bcl-2和Bax蛋白表达的变化。1-甲基-4-苯基吡啶离子（MPP）显著降低MES23.5细胞的细胞活力和线粒体膜电位。TF治疗可逆转这些作用。此外，TF预处理还可逆转MPP诱导的Bcl-2和Bax mRNA及蛋白表达的变化。这些数据表明，朝鲜淫羊藿的TF可保护小鼠免受MPTP诱导的多巴胺能神经元死亡以及多巴胺能MES23.5细胞免受MPP诱导的神经毒性。抗凋亡可能参与了这一过程。
